Metabolic and mitogenic effects of IGF-I and insulin on muscle cells of rainbow trout

被引:139
作者
Castillo, J [1 ]
Codina, M [1 ]
Martínez, ML [1 ]
Navarro, I [1 ]
Gutiérrez, J [1 ]
机构
[1] Univ Barcelona, Fac Biol, Dept Fisiol, E-08028 Barcelona, Spain
关键词
metabolic effects; proliferation;
D O I
10.1152/ajpregu.00459.2003
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
The relative function of IGF-I and insulin on fish muscle metabolism and growth has been investigated by the isolation and culture at different stages (myoblasts at day 1, myocytes at day 4, and myotubes at day 10) of rainbow trout muscle cells. This in vitro model avoids interactions with endogenous peptides, which could interfere with the muscle response. In these cells, the effects of IGF-I and insulin on cell proliferation, 2-deoxyglucose (2-DG), and L-alanine uptake at different development stages, and the use of inhibitors were studied and quantified. Insulin (10-1,000 nM) and IGF-I (10-100 nM) stimulated 2-DG uptake in trout myocytes at day 4 in a similar manner (maximum of 124% for insulin and of 142% for IGF-I), and this stimulation increased when cells differentiated to myotubes (maximum for IGF-I of 193%). When incubating the cells with PD-98059 and especially cytochalasin B, a reduction in 2-DG uptake was observed, suggesting that glucose transport takes place through specific facilitative transporters. IGF-I (1-100 nM) stimulated the L-alanine uptake in myocytes at day 4 (maximum of 239%), reaching higher values of stimulation than insulin (100-1,000 nM) (maximum of 160%). This stimulation decreased when cells developed to myotubes at day 10 (118% for IGF-I and 114% for insulin). IGF-I (0.125-25 nM) had a significant effect on myoblast proliferation, measured by thymidine incorporation (maximum of 170%), and required the presence of 2-5% fetal serum (FBS) to promote thymidine uptake. On the other hand, insulin was totally ineffective in stimulating thymidine uptake. We conclude that IGF-I is more effective than insulin in stimulating glucose and alanine uptake in rainbow trout myosatellite cells and that the degree of stimulation changes when cells differentiate to myotubes. IGF-I stimulates cell proliferation in this model of muscle in vitro and insulin does not. These results indicate the important role of IGF-I on growth and metabolism of fish muscle.
引用
收藏
页码:R935 / R941
页数:7
相关论文
共 62 条
  • [11] Acute IGF-I infusion stimulates protein synthesis in skeletal muscle and other tissues of neonatal pigs
    Davis, TA
    Fiorotto, ML
    Burrin, DG
    Vann, RC
    Reeds, PJ
    Nguyen, HV
    Beckett, PR
    Bush, JA
    [J]. AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM, 2002, 283 (04): : E638 - E647
  • [12] Comparison of recombinant barramundi and human insulin-like growth factor (IGF)-I in juvenile barramundi (Lates calcarifer):: In vivo metabolic effects, association with circulating IGF-binding proteins, and tissue localisation
    Degger, B
    Upton, Z
    Soole, K
    Collet, C
    Richardson, N
    [J]. GENERAL AND COMPARATIVE ENDOCRINOLOGY, 2000, 117 (03) : 395 - 403
  • [13] TNF-α impairs insulin signaling and insulin stimulation of glucose uptake in C2C12 muscle cells
    Del Aguila, LF
    Claffey, KP
    Kirwan, JP
    [J]. AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM, 1999, 276 (05): : E849 - E855
  • [14] Effect of vanadate and insulin on glucose transport in isolated adult rat cardiomyocytes
    Donthi, RV
    Huisamen, B
    Lochner, A
    [J]. CARDIOVASCULAR DRUGS AND THERAPY, 2000, 14 (05) : 463 - 470
  • [15] Characterization of an insulin-like growth factor (IGF) receptor and the insulin-like effects of IGF-1 in the bony fish, Lates calcarifer
    Drakenberg, K
    Carey, G
    Mather, P
    Anderson, A
    Sara, VR
    [J]. REGULATORY PEPTIDES, 1997, 69 (01) : 41 - 45
  • [16] EXPRESSION OF INSULIN-LIKE GROWTH-FACTOR-I IN NORMALLY AND ABNORMALLY DEVELOPING COHO SALMON (ONCORHYNCHUS-KISUTCH)
    DUAN, CM
    PLISETSKAYA, EM
    DICKHOFF, WW
    [J]. ENDOCRINOLOGY, 1995, 136 (02) : 446 - 452
  • [17] STIMULATION OF DNA-SYNTHESIS IN CHICKEN MUSCLE SATELLITE CELLS BY INSULIN AND INSULIN-LIKE GROWTH-FACTORS - EVIDENCE FOR EXCLUSIVE MEDIATION BY A TYPE-I INSULIN-LIKE GROWTH-FACTOR RECEPTOR
    DUCLOS, MJ
    WILKIE, RS
    GODDARD, C
    [J]. JOURNAL OF ENDOCRINOLOGY, 1991, 128 (01) : 35 - &
  • [18] REGULATION OF AMINO-ACID-TRANSPORT AND PROTEIN-METABOLISM IN MYOTUBES DERIVED FROM CHICKEN MUSCLE SATELLITE CELLS BY INSULIN-LIKE GROWTH FACTOR-I
    DUCLOS, MJ
    CHEVALIER, B
    GODDARD, C
    SIMON, J
    [J]. JOURNAL OF CELLULAR PHYSIOLOGY, 1993, 157 (03) : 650 - 657
  • [19] INSULIN-LIKE GROWTH FACTOR-I-STIMULATED GLUCOSE-TRANSPORT IN MYOTUBES DERIVED FROM CHICKEN MUSCLE SATELLITE CELLS
    DUCLOS, MJ
    CHEVALIER, B
    LEMARCHANDBRUSTEL, Y
    TANTI, JF
    GODDARD, C
    SIMON, J
    [J]. JOURNAL OF ENDOCRINOLOGY, 1993, 137 (03) : 465 - 472
  • [20] THE TYPE-II INSULIN-LIKE GROWTH-FACTOR (IGF) RECEPTOR HAS LOW AFFINITY FOR IGF-I ANALOGS - PLEIOTYPIC ACTIONS OF IGFS ON MYOBLASTS ARE APPARENTLY MEDIATED BY THE TYPE-I RECEPTOR
    EWTON, DZ
    FALEN, SL
    FLORINI, JR
    [J]. ENDOCRINOLOGY, 1987, 120 (01) : 115 - 123