Evaluation of antibacterial and toxicological activities of essential oil of Ocimum gratissimum L. and its major constituent eugenol

被引：17

作者：

Silva, Julio Cesar ^{[1
]}

Pereira, Raimundo Luiz Silva ^{[1
]}

de Freitas, Thiago Sampaio ^{[1
]}

Rocha, Janaina Esmeraldo ^{[1
]}

Macedo, Nair Silva ^{[1
]}

Nonato, Carla de Fatima Alves ^{[1
]}

Linhares, Marina Leite ^{[2
]}

Tavares, Daniely Sampaio Arruda ^{[3
]}

de Cunha, Francisco Assis Bezerra ^{[1
]}

Coutinho, Henrique Douglas Melo ^{[1
]}

de Lima, Sidney Goncalo ^{[4
]}

Pereira-Junior, Francisco Nascimento ^{[5
]}

Maia, Francisco Paulo Araujo ^{[6
]}

Neto, Ivo Cavalcante Pita ^{[6
]}

Rodrigues, Fabiola Fernandes Galvao ^{[1
]}

Santos, George Joaquim Garcia ^{[1
]}

机构：

[1] Reg Univ Cariri, Dept Biol Chem, R Cel Antonio Luis 1161, BR-63105000 Crato, CE, Brazil

[2] Univ Ctr Doutor Leao Sampaio, Ave Leao Sampaio 400, BR-63040000 Juazeiro Do Norte, CE, Brazil

[3] Univ Fed Ceara, Res & Dev Ctr Med, R Coronel Nunes de Melo 1000, BR-60430275 Fortaleza, CE, Brazil

[4] Univ Fed Piaui, Chem Dept, Campus Univ Minist Petronio Portella, BR-64049550 Teresina, PI, Brazil

[5] Fed Univ Cariri, Ctr Agr & Biodivers Sci CCAB, Crato, CE, Brazil

[6] Coll Dent, CECAPE, BR-63024015 Juazeiro De Norte, CE, Brazil

来源：

FOOD BIOSCIENCE | 2022年 / 50卷

关键词：

Antibacterial agents; Drosophila melanogaster; Eugenol; Ocimum gratisismumum L; essential oils; CHEMICAL-COMPOSITION; FRUIT-FLY; TOXICITY; MONOTERPENES;

D O I：

10.1016/j.fbio.2022.102128

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

The present study aimed to comparatively analyze the antibacterial activity, combined with antibiotics, and toxicological activity of the essential oil of O. gratissimum L. and its major compound, eugenol. The phyto-chemical analysis of the essential oil was performed by gas chromatography coupled with mass spectrometry. The minimum inhibitory concentration and combined antibiotic activity were performed by microdilution. Drosophila melanogaster was the model organism chosen to evaluate the toxicological response of substances. The chromatographic assay showed eugenol as the major compound of the essential oil of O. gratissimum L. The microbiological assays showed that in terms of antibacterial activity, both products showed similar activity, quite significant in Gram-negatives, such as those of strains of Pseudomonas aeruginosa. Similar results were verified in the modulatory activity, allowing the verification that the bioactivity performed by the essential oil of O. gratissimum L. is mainly influenced by its major constituent. Although the EC50 of the compounds varied subtly, the essential oil was less toxic compared to eugenol. The information obtained in the present study demonstrates that the essential oil of O. gratissimum L. is a promising antibacterial agent and better tolerated than its majority compound alone, in the model organism D. melanogaster.

引用

页数：8

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