Cloning of a full-length cDNA encoding ent-kaurene synthase from Gibberella fujikuroi:: Functional analysis of a bifunctional diterpene cyclase

被引：57

作者：

Toyomasu, T ^{[1
]}

Kawaide, H

Ishizaki, A

Shinoda, S

Otsuka, M

Mitsuhashi, W

Sassa, T

机构：

[1] Yamagata Univ, Dept Bioresource Engn, Yamagata 9908555, Japan

[2] RIKEN, Inst Phys & Chem Res, Frontier Res Program, Wako, Saitama 3510198, Japan

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2000年 / 64卷 / 03期

关键词：

diterpene cyclase; ent-kaurene synthase; Gibberella fujikuroi; gibberellin biosynthesis;

D O I：

10.1271/bbb.64.660

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We report here the nucleotide sequence of a full-length cDNA encoding ent-kaurene synthase that was isolated by a reverse-transcription polymerase chain reaction from Gibberella fujikuroi (Gcps/ks). This cDNA encodes 952 amino acid residues with a relative molecular mass of 107 kDa. The sequence similarity between Gcps/ks and ent-kaurene synthase of the gibberellin A(1)-producing fungus, Phaeosphaeria sp. L487, is very high, suggesting that Gcps/ks is also a bifunctional diterpene cyclase. Its recombinant protein expressed. in Escherichia coli converted geranylgeranyl diphosphate to copalyl diphosphate and ent-kaurene.

引用

页码：660 / 664

页数：5

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