Comparison of the SARS-CoV-2 Rapid antigen test to the real star Sars-CoV-2 RT PCR kit

被引：137

作者：

Kruettgen, Alexander ^{[1
]}

Cornelissen, Christian G. ^{[2
]}

Dreher, Michael ^{[2
]}

Hornef, Mathias W. ^{[3
]}

Imoehl, Matthias ^{[1
]}

Kleines, Michael ^{[1
]}

机构：

[1] Univ Hosp RWTH Aachen, Lab Diagnost Ctr, Pauwelsstr 30, D-52074 Aachen, Germany

[2] Univ Hosp RWTH Aachen, Dept Pneumol & Intens Care Med, Aachen, Germany

[3] Univ Hosp RWTH Aachen, Inst Med Microbiol, Aachen, Germany

来源：

JOURNAL OF VIROLOGICAL METHODS | 2021年 / 288卷

关键词：

COVID-19; Lateral flow assay; Antigen; SARS-CoV-2;

D O I：

10.1016/j.jviromet.2020.114024

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

There is an ongoing need for reliable antigen assays for timely and easy detection of individuals with acute SARS-CoV-2 infection. Using 75 swabs from patients previously tested positive by SARS-CoV-2 PCR and 75 swabs from patients previously tested negative by SARS-CoV-2 PCR, we investigated the sensitivity and specificity of the SARS-CoV-2 Rapid Antigen Test (Roche). We determined a specificity of 96 %. The assay's sensitivity with samples with a cycle threshold of < 25, 25-< 30, 30- <35, and > -35 was 100 %, 95 %, 44.8 % and 22.2 %, respectively. We conclude that sensitivity and specificity of the antigen assay is inferior to the PCR assay. However, the antigen assay may be a quick and easy to perform alternative for differentiation of individuals contagious for SARS-CoV-2 from non-contagious individuals.

引用

页数：2

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