The first extracellular domain of the tumour stem cell marker CD133 contains an antigenic ganglioside-binding motif

被引：69

作者：

Taieb, Nadira

Maresca, Marc

Guo, Xiao-Jun

Garmy, Nicolas

Fantini, Jacques

Yahi, Nouara ^{[1
]}

机构：

[1] Univ Aix Marseille 2, CRN2M, CNRS,UMR 6231, INRA,USC 2027,Lab Interact Mol & Syst Membranaire, F-13397 Marseille 20, France

来源：

CANCER LETTERS | 2009年 / 278卷 / 02期

关键词：

Prominin; Cancer stem cells; DNA sequencing; Glycolipid-binding domain; Ganglioside; GM1; CD133; Stem cells; Colon cancer cells; Glycolipid; HEMATOPOIETIC STEM; EPITHELIAL-CELLS; AC133; IDENTIFICATION; CHOLESTEROL; EXPRESSION; PROMININ; GENE; GALACTOSYLCERAMIDE; MICRODOMAINS;

D O I：

10.1016/j.canlet.2009.01.013

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Prominin 1/CD133 is a marker of transplantable cancer stem cells. We have generated antipeptide antibodies against a N-terminal epitope of CD133 belonging to a ganglioside-binding domain. The labelling of colon cancer cells with these antibodies was inhibited by various gangliosides including GM1 and GD3, but not GT1b. CD133 immunolabelling progressively decreased to undetectable levels in post-confluent cultures, possibly through ganglioside-mediated epitope masking since the staining was partially recovered after chemical disruption of lipid rafts. We suggest that selected gangliosides could modulate the accessibility of CD133 and regulate cell-cell contacts involving CD133* stem cells at the earliest steps of tumour development. (C) 2009 Elsevier Ireland Ltd. All rights reserved.

引用

页码：164 / 173

页数：10

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