Application of Dispersive Liquid-Liquid Microextraction Technique for the Analysis of Norfloxacin Antibiotic in Chicken Breast (Pectoralis Major) by High Performance Liquid Chromatography With Fluorescence Detector

Brazil is considered the second largest producer and the first largest exporter of chicken meat in the world. With the objective of guaranteeing the efficiency of this expanding productive demand, veterinary antibiotics have been used intensively, however, its indiscriminate use has favored the emergence of bacteria resistant to man. In this work, a method for the determination of norfloxacin (NOR), a fluoroquinolone antibiotic (FQ) was applied in chicken breast (Pectoralis major) by dispersive liquid-liquid microextraction (DLLME) associated with high-performance liquid chromatography with fluorescence detector (HPLC-FL). The results were satisfactory, with a coefficient of determination of 0.991 to 0.998. Precision expressed as relative standard deviation (DPR %) ranged from 0.2% to 3.6%. Recovery rates ranged from 90.3% to 111.5 %. The limit of detection and limit of quantification were respectively 1.4 and 4.7 mu g.kg-1. In this way, the method can be considered precise, accurate and reliable for determinations of real samples in routine analysis.