Propolis Modulates Fibronectin Expression in the Matrix of Thermal Injury

被引:36
作者
Olczyk, Pawel [1 ]
Komosinska-Vassev, Katarzyna [2 ]
Wisowski, Grzegorz [2 ]
Mencner, Lukasz [2 ]
Stojko, Jerzy [3 ]
Kozma, Ewa M. [2 ]
机构
[1] Med Univ Silesia, Dept Community Pharm, PL-41200 Sosnowiec, Poland
[2] Med Univ Silesia, Dept Clin Chem & Lab Diagnost, PL-41200 Sosnowiec, Poland
[3] Med Univ Silesia, Ctr Med Expt, PL-40752 Katowice, Poland
关键词
VENOUS LEG ULCERS; HIGH-VOLTAGE STIMULATION; LEVEL LASER THERAPY; SILVER SULFADIAZINE; HISTOPATHOLOGICAL EVALUATION; ELECTRICAL-STIMULATION; 2ND-DEGREE BURNS; ULTRASOUND; PROTEINS; EFFICACY;
D O I
10.1155/2014/748101
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The aim of the study was to assess the propolis effect on fibronectin metabolism in the course of burn wounds healing process. A model of burn wound healing of pig skin was applied. The amount of the released glycoprotein was assessed by a surface plasmon resonance. The profile of extracted fibronectin components was also assessed by an electrophoresis in polyacrylamide gel, with a subsequent immunodetection by Western Blotting. Propolis burn treatment decreased the release of fibronectin components from healing wounds in relation to damages treated with silver sulfadiazine. The main reason of decreased extraction of fibronectin components from wounds treated with propolis was a substantial decrease of degradation product release of the mentioned glycoprotein, which was observed particularly from the 3rd to 5th day of the repair. Wounds treatment with propolis demonstrated, especially in relation to damages treated with silver sulfadiazine, the decreased release of synthesized fibronectin molecules. The obtained results suggest that propolis modifies fibronectin metabolism in the course of wound healing process. The influence of propolis is reflected in prevention of fibronectin biosynthesis as well as its degradation in the wound area. The above-mentioned metabolic changes may decrease the risk of complications in the repair wounds process.
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页数:10
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