Deep-UV laser-based fluorescence lifetime imaging microscopy of single molecules

被引：35

作者：

Li, Q ^{[1
]}

Ruckstuhl, T ^{[1
]}

Seeger, S ^{[1
]}

机构：

[1] Univ Zurich, Inst Phys Chem, CH-8057 Zurich, Switzerland

来源：

JOURNAL OF PHYSICAL CHEMISTRY B | 2004年 / 108卷 / 24期

关键词：

D O I：

10.1021/jp0375160

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Deep ultraviolet (deep-UV) laser-based fluorescence lifetime imaging microscopy of single dye molecules adsorbed on quartz glass surfaces is presented. The instrumental setup uses a mode-locked diode-pumped picosecond laser emitting at 266 nm with a repetition rate of 40 MHz. Applying the time-resolved single-photon counting method, we investigated the laser dye 2,2"'-dimethyl-p-quaterphenyl and obtained the fluorescence intensity and fluorescence lifetime images of individual adsorbed molecules. The described setup is also well-suited for biological applications for ultrasensitive detection of intrinsic fluorescence after UV excitation.

引用

页码：8324 / 8329

页数：6

共 37 条

[1]

Böhmer M, 2001, REV SCI INSTRUM, V72, P4145, DOI 10.1063/1.1406926

[2] Single-molecule identification of Coumarin-120 by time-resolved fluorescence detection: Comparison of one- and two-photon excitation in solution [J].

Brand, L ;

Eggeling, C ;

Zander, C ;

Drexhage, KH ;

Seidel, CAM .

JOURNAL OF PHYSICAL CHEMISTRY A, 1997, 101 (24) :4313-4321

[3] On/off blinking and switching behaviour of single molecules of green fluorescent protein [J].

Dickson, RM ;

Cubitt, AB ;

Tsien, RY ;

Moerner, WE .

NATURE, 1997, 388 (6640) :355-358

[4]

GERRITSEN HC, 1994, P SOC PHOTO-OPT INS, V2329, P260

[5] FLUORESCENCE LIFETIME MEASUREMENTS USING A NOVEL FIBEROPTIC LASER SCANNING CONFOCAL MICROSCOPE [J].

GHIGGINO, KP ;

HARRIS, MR ;

SPIZZIRRI, PG .

REVIEW OF SCIENTIFIC INSTRUMENTS, 1992, 63 (05) :2999-3002

[6] PHOTOPHYSICAL PROPERTIES AND LASER PERFORMANCE OF PHOTOSTABLE UV LASER-DYES .3. STERICALLY HINDERED P-QUATERPHENYLS [J].

GUSTEN, H ;

RINKE, M ;

WIRTH, HO .

APPLIED PHYSICS B-PHOTOPHYSICS AND LASER CHEMISTRY, 1988, 45 (04) :279-284

[7] High-resolution colocalization of single dye molecules by fluorescence lifetime imaging microscopy [J].

Heilemann, M ;

Herten, DP ;

Heintzmann, R ;

Cremer, C ;

Muller, C ;

Tinnefeld, P ;

Weston, KD ;

Wolfrum, J ;

Sauer, M .

ANALYTICAL CHEMISTRY, 2002, 74 (14) :3511-3517

[8] Excitonic behavior of rhodamine dimers:: A single-molecule study [J].

Hernando, J ;

van der Schaaf, M ;

van Dijk, EMHP ;

Sauer, M ;

García-Parajó, MF ;

van Hulst, NF .

JOURNAL OF PHYSICAL CHEMISTRY A, 2003, 107 (01) :43-52

[9] Orientation dependence of fluorescence lifetimes near an interface [J].

Kreiter, M ;

Prummer, M ;

Hecht, B ;

Wild, UP .

JOURNAL OF CHEMICAL PHYSICS, 2002, 117 (20) :9430-9433

[10] Sizing of single fluorescently stained DNA fragments by scanning microscopy [J].

Laib, S ;

Rankl, M ;

Ruckstuhl, T ;

Seeger, S .

NUCLEIC ACIDS RESEARCH, 2003, 31 (22) :e138

← 1 2 3 4 →