A METTL3-METTL14 complex mediates mammalian nuclear RNA N6-adenosine methylation

被引：2715

作者：

Liu, Jianzhao ^{[1
,2
]}

Yue, Yanan ^{[1
,2
]}

Han, Dali ^{[1
,2
]}

Wang, Xiao ^{[1
,2
]}

Fu, Ye ^{[1
,2
]}

Zhang, Liang ^{[1
,2
]}

Jia, Guifang ^{[1
,2
]}

Yu, Miao ^{[1
,2
]}

Lu, Zhike ^{[1
,2
]}

Deng, Xin ^{[1
,2
]}

Dai, Qing ^{[1
,2
]}

Chen, Weizhong ^{[1
,2
]}

He, Chuan ^{[1
,2
]}

机构：

[1] Univ Chicago, Dept Chem, Chicago, IL 60637 USA

[2] Univ Chicago, Inst Biophys Dynam, Chicago, IL 60637 USA

来源：

NATURE CHEMICAL BIOLOGY | 2014年 / 10卷 / 02期

基金：

美国国家卫生研究院;

关键词：

MESSENGER-RNA; N6-METHYLADENOSINE; PURIFICATION; SUBUNIT; PROTEIN; IME4;

D O I：

10.1038/nchembio.1432

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

N-6-methyladenosine (m(6)A) is the most prevalent and reversible internal modification in mammalian messenger and noncoding RNAs. We report here that human methyltransferase-like 14 (METTL14) catalyzes m(6)A RNA methylation. Together with METTL3, the only previously known m(6)A methyltransferase, these two proteins form a stable heterodimer core complex of METTL3-METTL14 that functions in cellular m(6)A deposition on mammalian nuclear RNAs. WTAP, a mammalian splicing factor, can interact with this complex and affect this methylation.

引用

页码：93 / 95

页数：3

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