Method to determination of total residues of the insecticide acetamiprid and its metabolites in crops by gas chromatography

An analytical method has been developed for the determination of acetamiprid, (E)-N-1-[(6-chloro-3-pyridyl)methyl]-N-2-cyano-N-1- methylacetamidine (ATP) and its metabolites, IM-2-1, IM-0, IC-0 and IM-0-Glucose in crops by gas chromatography (GC). ATP and its metabolites in crops were extracted with methanol and derivatized to methyl 6-chloronicotinate (IC-0-Me) through alkali hydrolysis, potassium permanganate oxidation and then esterification by diazomethane, followed by column chromatography clean-up and GC determination. The limit of detection was 0.01 ppm and the recoveries of fortified samples ranged from 74 to 92%.