Atomic Force Microscopy Observation of Membrane Proteins Suspended over Carbon Nanotube Network

被引：7

作者：

Sumitomo, Koji ^{[1
]}

Shinozaki, Youichi ^{[1
]}

Takagi, Daisuke ^{[1
]}

Nakashima, Hiroshi ^{[1
]}

Kobayashi, Yoshihiro ^{[1
]}

Torimitsu, Keiichi ^{[1
]}

机构：

[1] NTT Corp, NTT Basic Res Labs, Kanagawa 2430198, Japan

来源：

JAPANESE JOURNAL OF APPLIED PHYSICS | 2009年 / 48卷 / 08期

基金：

日本学术振兴会; 日本科学技术振兴机构;

关键词：

BACTERIORHODOPSIN; GROWTH; NANOPARTICLES; AFM;

D O I：

10.1143/JJAP.48.08JB18

中图分类号：

O59 [应用物理学];

学科分类号：

摘要：

Atomic force microscopy (AFM) imaging of membrane proteins suspended over a nanostructure in liquid is a promising way to understand the structure and function of working proteins, although the membrane deformation that occurs during scanning makes it difficult to obtain a high resolution image. This study proposes an artificial cell system for the AFM observation of functional membrane proteins that consists of a sub-micron well on Si, a biological membrane, and a carbon nanotube (CNT) network. We successfully observed molecular-scale images of a purple membrane suspended over sub-micron well patterns. By using a CNT network to hold the suspended membrane, we suppressed the membrane deformation caused by the "imaging force". The CNT network takes the place of a cytoskeleton in supporting the cell membrane suspended over the well thus improving the spatial resolution of AFM measurement. (C) 2009 The Japan Society of Applied Physics

引用

页数：4

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