NMDA-dependent, but not group I metabotropic glutamate receptor-dependent, long-term depression at schaffer collateral-CA1 synapses is associated with long-term reduction of release from the rapidly recycling presynaptic vesicle pool

被引:34
作者
Zhang, Xiao-lei
Zhou, Zhen-yu
Winterer, Jochen
Mueller, Wolfgang
Stanton, Patric K. [1 ]
机构
[1] New York Med Coll, Dept Cell Biol & Anat, Valhalla, NY 10595 USA
[2] Humboldt Univ, Charite, Dept Psychiat, D-10117 Berlin, Germany
[3] Univ New Mexico, Sch Med, Dept Neurol, Albuquerque, NM 87131 USA
[4] Univ New Mexico, Sch Med, Dept Neurosci, Albuquerque, NM 87131 USA
[5] Univ New Mexico, Sch Med, Dept Neurosurg, Albuquerque, NM 87131 USA
关键词
CA1; hippocampus; long-term depression; metabotropic glutamate receptors; NMDA; presynaptic; rapidly recycling vesicle pool; Schaffer collateral; transmitter release;
D O I
10.1523/JNEUROSCI.3091-06.2006
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Postsynaptic alterations have been suggested to account for NMDA receptor (NMDAR)-dependent long-term depression (LTD) and long-term potentiation of synaptic strength, although there is substantial evidence supporting changes in presynaptic release. Direct chemical activation of either NMDA or group I metabotropic glutamate receptor (mGluR1) elicits LTD of similar magnitudes, but it is unknown whether they share common expression mechanisms. Using dual-photon laser-scanning microscopy of FM1-43 [N-(3-triethylammoniumpropyl)- 4-(4-(dibutylamino)styryl) pyridinium dibromide] to directly visualize presynaptic vesicular release from the rapidly recycling vesicle pool (RRP) at Schaffer collateral terminals in field CA1 of rat hippocampal slices, we found that a persistent reduction in vesicular release from the RRP is induced by NMDA-LTD but not by mGluR1-LTD. Variance-mean analyses of Schaffer collateral release probability (P-r) at varying extracellular calcium concentrations confirmed that NMDA-LTD was associated with reduced P-r, whereas mGluR1-LTD was not. Pharmacological isolation of NMDAR-dependent and mGluR-dependent forms of stimulus-evoked LTD revealed that both are composed of a combination of presynaptic and postsynaptic alterations. However, when group I mGluR-dependent LTD was isolated by combining an NMDAR blocker with a group II mGluR antagonist, this form of LTD was purely postsynaptic. The nitric oxide synthase inhibitor N omega-nitro-L-arginine blocked the induction of NMDA-LTD but did not alter mGluR-LTD, consistent with a selective role for nitric oxide as a retrograde messenger mediating NMDA-LTD. These data demonstrate that single synapses can express multiple forms of LTD with different sites of expression, that NMDA-LTD is a combination of presynaptic and postsynaptic alterations, but that group I mGluR-LTD appears to be expressed entirely postsynaptically.
引用
收藏
页码:10270 / 10280
页数:11
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