Broad range RT-PCR assays targeting human noroviruses also detect swine noroviruses

被引：7
作者：
L'Homme, Yvan [1 ]
Sansregret, Raphael [1 ]
Simard, Carole [1 ]
机构：
[1] Canadian Food Inspect Agcy, St Hyacinthe Lab, St Hyacinthe, PQ J2S 8E3, Canada
来源：
FOOD MICROBIOLOGY | 2009年 / 26卷 / 05期
关键词：
RT-PCR; Norovirus; Real time RT-PCR; Food diagnostics; REVERSE TRANSCRIPTION-PCR; ENTERIC CALICIVIRUSES; GASTROENTERITIS OUTBREAK; UNITED-STATES; PRIMER PAIR; VIRUSES; CONSUMPTION; DIAGNOSIS; SHELLFISH;
D O I：
10.1016/j.fm.2009.03.011
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Conventional broad range RT-PCR and real time RT-PCR assays designed to detect human noroviruses (NoVs) also efficiently detect swine NoVs. Investigation of the primers and probe binding sites revealed strong homologies between swine NoVs genomic sequences and human primer sequences. These findings have a serious impact on food diagnostic methods and laboratories. Crown Copyright (c) 2009 Published by Elsevier Ltd. All rights reserved.
引用
收藏
页码：552 / 555
页数：4
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