Enhanced gas chromatography-mass spectrometry method for bacterial polyhydroxyalkanoates analysis

被引：44

作者：

Tan, Giin-Yu Amy ^{[1
,2
]}

Chen, Chia-Lung ^{[1
]}

Ge, Liya ^{[1
]}

Li, Ling ^{[1
]}

Wang, Lin ^{[1
,2
]}

Zhao, Lei ^{[1
,2
]}

Mo, Yu ^{[1
,2
]}

Tan, Swee Ngin ^{[3
]}

Wang, Jing-Yuan ^{[1
,2
]}

机构：

[1] Nanyang Technol Univ, Nanyang Environm & Water Res Inst, Residues & Resource Reclamat Ctr, Singapore 637141, Singapore

[2] Nanyang Technol Univ, Sch Civil & Environm Engn, Div Environm & Water Resources Engn, Singapore 639798, Singapore

[3] Nanyang Technol Univ, Nat Sci & Sci Educ Acad Grp, Singapore 637616, Singapore

来源：

JOURNAL OF BIOSCIENCE AND BIOENGINEERING | 2014年 / 117卷 / 03期

关键词：

Gas chromatography; Mass spectrometry; Polyhydroxyalkanoate; 3-Hydroxyalkanoic acid; Homologous; MEDIUM-CHAIN-LENGTH; UNSATURATED MONOMERS; ALKANES; SYSTEMS;

D O I：

10.1016/j.jbiosc.2013.08.020

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A gas chromatography-mass spectrometry method for quantification of polyhydroxyalkanoates (PHAs), containing 4-carbon to 16-carbon monomers, even in the absence of standards, was developed. Strong linear correlations existed between PHA carbon number and retention time/response factor (R-2 >= 0.987). Based on the correlations, high recovery values, between 100.5% and 114.3%, were obtained for PHA polymers. (C) 2013, The Society for Biotechnology, Japan. All rights reserved.

引用

页码：379 / 382

页数：4

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