Production and characterization of a microbial glucan, synthesized by Geobacillus tepidamans V264 isolated from Bulgarian hot spring

被引：79

作者：

Kambourova, Margarita ^{[2
]}

Mandeva, Rossica ^{[2
]}

Dimova, Damyana ^{[2
]}

Poli, Annarita ^{[1
]}

Nicoaus, Barbara ^{[1
]}

Tommonaro, Giuseppina ^{[1
]}

机构：

[1] ICB CNR, Inst Biomol Chem, I-80078 Pozzuoli, NA, Italy

[2] Bulgarian Acad Sci, Dept Microbiol, BU-1113 Sofia, Bulgaria

来源：

CARBOHYDRATE POLYMERS | 2009年 / 77卷 / 02期

关键词：

Exopolysaccharide (EPS); Geobacillus tepidamans; Microbial glucan; Polysaccharide thermostability; Anti-toxic activity; EXOPOLYSACCHARIDE PRODUCTION; HYDROTHERMAL VENTS; SHALLOW; POLYSACCHARIDE; BACTERIUM;

D O I：

10.1016/j.carbpol.2009.01.004

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

The thermophilic strain producing extracellular polysaccharide (EPS) was isolated from Velingrad hot spring, Bulgaria. StrainV264 showed 98% similarity in 16S rRNA gene sequence with Geobacillus tepidamans. Maltose was found to be the most appropriate carbon source for exopolysaccharide production. Maximum EPS production was reached in the early stationary phase. Highest EPS production of 111.4 mg L-1 was obtained in fermentor batch cultures at 300 rpm. The purified EPS expressed a high molecular weight and it was very stable at high temperature, showing a degradation at 280 degrees C. Chemical composition of the bio-polymer, determined by using a high pressure anion exchange-pulsed amperometric detector (HPAE-PAD), showed glucose as its major component. The polysaccharide was also investigated by spectroscopic methods (H-1 and C-13 NMR) which demonstrated the structure complexity of this biopolymer. (C) 2009 Elsevier Ltd. All rights reserved.

引用

页码：338 / 343

页数：6

共 22 条

[1] Antiviral and immunoregulatory effect of a novel exopolysaccharide from a marine thermotolerant Bacillus licheniformis [J].

Arena, A ;

Maugeri, TL ;

Pavone, B ;

Iannello, D ;

Gugliandolo, C ;

Bisignano, G .

INTERNATIONAL IMMUNOPHARMACOLOGY, 2006, 6 (01) :8-13

[2] A NEW BIOACTIVE DERIVATIVE OF AVAROL FROM THE MARINE SPONGE DYSIDEA-AVARA [J].

CRISPINO, A ;

DEGIULIO, A ;

DEROSA, S ;

STRAZZULLO, G .

JOURNAL OF NATURAL PRODUCTS, 1989, 52 (03) :646-648

[3] A polysaccharide from tomato (Lycopersicon esculentum) peels affects NF-κB activation in LPS-Stimulated J774 macrophages [J].

De Stefano, Daniela ;

Tommonaro, Giuseppina ;

Simeon, Vittorio ;

Poli, Annarita ;

Nicolaus, Barbara ;

Carnuccio, Rosa .

JOURNAL OF NATURAL PRODUCTS, 2007, 70 (10) :1636-1639

[4] A COLORIMETRIC METHOD FOR THE DETERMINATION OF SUGARS [J].

DUBOIS, M ;

GILLES, K ;

HAMILTON, JK ;

REBERS, PA ;

SMITH, F .

NATURE, 1951, 168 (4265) :167-167

[5]

Finney D.J., 1977, PROBIT ANAL, VIII

[6] Deep-sea hydrothermal vents: A new source of innovative bacterial exopolysaccharides of biotechnological interest [J].

Guezennec, J .

JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY, 2002, 29 (04) :204-208

[7] Influence of nutritional factors on the nature, yield, and composition of exopolysaccharides produced by Gluconacetobacter xylinus I-2281 [J].

Kornmann, H ;

Duboc, P ;

Marison, I ;

von Stockar, U .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2003, 69 (10) :6091-6098

[8] Chemical composition of two exopolysaccharides from Bacillus thermoantarcticus [J].

Manca, MC ;

Lama, L ;

Improta, R ;

Esposito, E ;

Gambacorta, A ;

Nicolaus, B .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1996, 62 (09) :3265-3269

[9]

MINALE L, 1974, TETRAHEDRON LETT, P3401

[10] Purification and characterization of a novel polysaccharide involved in the pellicle produced by a thermotolerant Acetobacter strain [J].

Moonmangmee, S ;

Toyama, H ;

Adachi, O ;

Theeragool, G ;

Lotong, N ;

Matsushita, K .

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 2002, 66 (04) :777-783

← 1 2 3 →