Evaluation of response of dynamics change in bioaugmentation process in diesel-polluted seawater via high-throughput sequencing: Degradation characteristic, community structure, functional genes

被引:41
作者
Xue Jianliang [1 ]
Shi Ke [1 ]
Chen Chuan [2 ]
Bai Yu [3 ]
Cui Qinqin [4 ]
Li Nana [1 ]
Fu Xinge [1 ]
Qiao Yanlu [1 ]
机构
[1] Shandong Univ Sci & Technol, Coll Safety & Environm Engn, Qingdao 266590, Shandong, Peoples R China
[2] Harbin Inst Technol, Sch Environm, Haerbin 150001, Heilongjiang, Peoples R China
[3] Chinaunicom Syst Integrat Co Ltd, 131 Xidan North Rd, Beijing 100085, Peoples R China
[4] Qingdao Binhai Univ, Sch Architecture & Engn, Qingdao 266555, Shandong, Peoples R China
关键词
High-throughput sequencing; Microbial community; Recovery; Bioaugmentation; Functional genes; PETROLEUM-DEGRADING BACTERIA; OIL-SPILL; MARINE ENVIRONMENTS; BIOREMEDIATION; HYDROCARBON; BIOSTIMULATION; GULF; STRATEGIES; MECHANISM;
D O I
10.1016/j.jhazmat.2020.123569
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
Identification of microorganisms that contribute to the whole microbial community is important. In this study, dynamic changes in bioaugmentation process in diesel-polluted seawater collected from two different sites were assessed via simulation experiments. Ultraviolet spectrophotometry and analysis using the molecular operating environment software revealed that the degradation rate of diesel due to bioaugmentation was higher than 70 % after 45 days because of the formation of hydrogen bonds among biosurfactants and diesel components. Community structure and functional genes were analysed via high-throughput sequencing. Results showed that community diversity recovered during bioaugmentation. Principal coordinate analysis showed that the difference in microbial community between the two sites was considerably smaller than that when diesel was added and bioaugmentation was conducted. After bioaugmentation, the main families playing key roles in degradation that became dominant were Alcanivoracaceae, Rhodobiaceae, and Rhodospirillaceae. Moreover, the abundance of functional genes remarkably increased at two different sites.
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页数:8
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