Purification and characterization of lectin from Lathyrus sativus

被引：2
|
作者：
Sawhney, VM [1 ]
Santha, IM [1 ]
Timko, MP [1 ]
Mehta, SL [1 ]
机构：
[1] INDIAN AGR RES INST, DIV BIOCHEM, NEW DELHI 110012, INDIA
来源：
JOURNAL OF PLANT BIOCHEMISTRY AND BIOTECHNOLOGY | 1996年 / 5卷 / 02期
关键词：
Lathyrus sativus; lectin; molecular characterization; carbohydrate specificity; amino acid sequence;
D O I：
10.1007/BF03262989
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Lectin has been isolated and purified from Lathyrus sativus using ammonium sulphate precipitation followed by affinity chromatography. The molecular weight as determined by HPLC was found to be 42kD. The lectin is a tetramer, consisting of two types of subunits of which the heavier subunit consists of 2 polypeptides of mol wt of about 21 kD and 16 kD while the smaller subunits consists of two polypeptides of about 5kD as. revealed by SDS-PAGE. The most potent sugar inhibitor of the Lathyrus lectin was found to be alpha-methyl D-mannoside. The N-terminal amino add sequence was similar to that of pea lectin sequence.
引用
收藏
页码：91 / 95
页数：5
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