A LC-MS-based workflow for measurement of branched fatty acid esters of hydroxy fatty acids

被引：70

作者：

Zhang, Tejia ^{[1
]}

Chen, Shili ^{[1
]}

Syed, Ismail ^{[2
,3
]}

Stahlman, Marcus ^{[4
]}

Kolar, Matthew J. ^{[1
]}

Homan, Edwin A. ^{[5
]}

Chu, Qian ^{[1
]}

Smith, Ulf ^{[6
]}

Boren, Jan ^{[4
]}

Kahn, Barbara B. ^{[2
,3
]}

Saghatelian, Alan ^{[1
]}

机构：

[1] Salk Inst Biol Studies, 10010 N Torrey Pines Rd, La Jolla, CA 92037 USA

[2] Beth Israel Deaconess Med Ctr, Dept Med, Div Endocrinol Diabet & Metab, Boston, MA 02215 USA

[3] Harvard Univ, Sch Med, Boston, MA USA

[4] Univ Gothenburg, Dept Mol & Clin Med, Wallenberg Lab, Gothenburg, Sweden

[5] Harvard Univ, Dept Chem & Chem Biol, Cambridge, MA 02138 USA

[6] Univ Gothenburg, Dept Mol & Clin Med, Lundberg Lab Diabet Res, Gothenburg, Sweden

来源：

NATURE PROTOCOLS | 2016年 / 11卷 / 04期

基金：

美国国家卫生研究院; 瑞典研究理事会;

关键词：

QUANTITATIVE-ANALYSIS; INSULIN-RESISTANCE; LINKING OBESITY; EXTRACTION; CHROMATOGRAPHY; METABOLOMICS; LIPIDS; METABOLITES; STEROLS;

D O I：

10.1038/nprot.2016.040

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Branched fatty acid esters of hydroxy fatty acids (FAHFAs) are a recently discovered class of endogenous mammalian lipids with antidiabetic and anti-inflammatory effects. We previously identified 16 different FAHFA families, such as branched palmitic acid esters of hydroxy stearic acids (PAHSAs); each family consists of multiple isomers in which the branched ester is at different positions (e.g., 5- and 9-PAHSA). We anticipate increased need for PAHSA measurements as markers of metabolic and inflammatory health. In this protocol, we provide a detailed description of the extraction of FAHFAs from human or mouse tissues, their enrichment by solid-phase extraction and subsequent analysis by LCLC-MS. For a sample size of 6-12, the time frame is 2-3 d.

引用

页码：747 / 763

页数：17

共 33 条

[1] Adipose-selective targeting of the GLUT4 gene impairs insulin action in muscle and liver [J].

Abel, ED ;

Peroni, O ;

Kim, JK ;

Kim, YB ;

Boss, O ;

Hadro, E ;

Minnemann, T ;

Shulman, GI ;

Kahn, BB .

NATURE, 2001, 409 (6821) :729-733

[2] Sample preparation and orthogonal chromatography for broad polarity range plasma metabolomics: Application to human subjects with neurodegenerative dementia [J].

Armirotti, Andrea ;

Basit, Abdul ;

Realini, Natalia ;

Caltagirone, Carlo ;

Bossu, Paola ;

Spalletta, Gianfranco ;

Piomelli, Daniele .

ANALYTICAL BIOCHEMISTRY, 2014, 455 :48-54

[3] Separation and quantitation of water soluble cellular metabolites by hydrophilic interaction chromatography-tandem mass spectrometry [J].

Bajad, Sunil U. ;

Lu, Wenyun ;

Kimball, Elizabeth H. ;

Yuan, Jie ;

Peterson, Celeste ;

Rabinowitz, Joshua D. .

JOURNAL OF CHROMATOGRAPHY A, 2006, 1125 (01) :76-88

[4]

BLIGH EG, 1959, CAN J BIOCHEM PHYS, V37, P911

[5] Lipids of human meibum: mass-spectrometric analysis and structural elucidation [J].

Butovich, Igor A. ;

Uchiyama, Eduardo ;

McCulley, James P. .

JOURNAL OF LIPID RESEARCH, 2007, 48 (10) :2220-2235

[6] Human tear film and meibum. Very long chain wax esters and (O-acyl)-omega-hydroxy fatty acids of meibum [J].

Butovich, Igor A. ;

Wojtowicz, Jadwiga C. ;

Molai, Mike .

JOURNAL OF LIPID RESEARCH, 2009, 50 (12) :2471-2485

[7] The worldwide epidemiology of type 2 diabetes mellitus-present and future perspectives [J].

Chen, Lei ;

Magliano, Dianna J. ;

Zimmet, Paul Z. .

NATURE REVIEWS ENDOCRINOLOGY, 2012, 8 (04) :228-236

[8]

Cox David M, 2005, J Biomol Tech, V16, P83

[9] Adipocyte dysfunctions linking obesity to insulin resistance and type 2 diabetes [J].

Guilherme, Adilson ;

Virbasius, Joseph V. ;

Puri, Vishwajeet ;

Czech, Michael P. .

NATURE REVIEWS MOLECULAR CELL BIOLOGY, 2008, 9 (05) :367-377

[10] A novel ChREBP isoform in adipose tissue regulates systemic glucose metabolism [J].

Herman, Mark A. ;

Peroni, Odile D. ;

Villoria, Jorge ;

Schoen, Michael R. ;

Abumrad, Nada A. ;

Blueher, Matthias ;

Klein, Samuel ;

Kahn, Barbara B. .

NATURE, 2012, 484 (7394) :333-U66

← 1 2 3 4 →