Oral MucoRice expressing double-mutant cholera toxin A and B subunits induces toxin-specific neutralising immunity

被引：31

作者：

Yuki, Yoshikazu ^{[1
]}

Tokuhara, Daisuke ^{[1
]}

Nochi, Tomonori ^{[1
]}

Yasuda, Hiroshi ^{[2
]}

Mejima, Mio ^{[1
]}

Kurokawa, Shiho ^{[1
]}

Takahashi, Yuko ^{[1
]}

Kataoka, Nobuhiro ^{[1
]}

Nakanishi, Ushio ^{[1
]}

Hagiwara, Yukari ^{[3
]}

Fujihashi, Kohtaro ^{[4
]}

Takaiwa, Fumio ^{[5
]}

Kiyono, Hiroshi ^{[1
,4
]}

机构：

[1] Univ Tokyo, Inst Med Sci, Tokyo 1088639, Japan

[2] Natl Agr Res Ctr Hokkaido Reg, Sapporo, Hokkaido 0628555, Japan

[3] Biol Res Ctr, Kitasato Inst, Saitama 3640026, Japan

[4] Univ Alabama, Immunobiol Vaccine Ctr, Dept Pediat Dent, Birmingham, AL 35294 USA

[5] Natl Inst Agrobiol Sci, Tsukuba, Ibaraki 3058602, Japan

来源：

VACCINE | 2009年 / 27卷 / 43期

基金：

日本学术振兴会;

关键词：

Rice-based vaccine; MucoRice; Oral vaccine; Cholera; Cold-chain-free; Mutant cholera toxin; PLANT-BASED VACCINES; ADP-RIBOSYLTRANSFERASE; FUSION PROTEIN; RICE; MUCOSAL; GENE; ADJUVANTICITY; RESPONSES; SEQUENCE; SEED;

D O I：

10.1016/j.vaccine.2009.07.071

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Rice-expressed cholera toxin B (CTB) subunit is a cold-chain-free oral vaccine that effectively induces enterotoxin-neutralising immunity. We created another rice-based vaccine, MucoRice, expressing nontoxic double-mutant cholera toxin (dmCT) with CTA and CTB subunits. Western-blot analysis suggested that MucoRice-dmCT had the shape of a multicomponent vaccine. Oral administration of MucoRice-dmCT induced CTB- but not CTA-specific serum IgG and mucosal IgA antibodies, generating protective immunity against cholera toxin without inducing rice-protein-specific antibody responses. The potency of MucoRice-dmCT was equal to that of MucoRice-CTB vaccine. MucoRice has the potential to be used as a safe multicomponent vaccine expression system. (C) 2009 Elsevier Ltd. All rights reserved.

引用

页码：5982 / 5988

页数：7

共 21 条

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