A Label-free and Turn-on Fluorescence Strategy for Kanamycin Detection Based on the NMM/G-quadruplex Structure

被引：15

作者：

Yang, Hualin ^{[1
]}

Wu, Qinghua ^{[2
]}

Su, Dongxiao ^{[1
]}

Wang, Yun ^{[1
]}

Li, Li ^{[1
]}

Zhang, Xingping ^{[1
]}

机构：

[1] Yangtze Univ, Jingchu Food Res & Dev Ctr, Coll Life Sci, Jingzhou 434025, Hubei, Peoples R China

[2] Univ Hradec Kralove, Dept Chem, Fac Sci, Hradec Kralove, Czech Republic

来源：

ANALYTICAL SCIENCES | 2017年 / 33卷 / 02期

基金：

中国国家自然科学基金;

关键词：

Kanamycin detection; G-quadruplex structure; DNA aptamer; METHYL MESOPORPHYRIN IX; IN-VITRO SELECTION; AMINOGLYCOSIDE ANTIBIOTICS; DNA; DERIVATIZATION; CHROMATOGRAPHY; APTASENSOR; MOLECULES; APTAMERS; SEQUENCE;

D O I：

10.2116/analsci.33.133

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

In the present work, a simple design of a "label-free" fluorescence method for kanamycin was developed and explored based on a specific DNA aptamer as a recognizer and a NMM/G-quadruplex DNA system as a reporter. The titration experiment showed a linear detection range of 0.5 to 100 nM with a low detection limit of 0.5 nM. It also showed excellent selectivity in a selectivity experiment. It was then successfully employed to detect kanamycin in milk with a excellent reliability.

引用

页码：133 / 135

页数：3

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