Health-promoting compounds of broccoli (Brassica oleracea L. var. italica) plants as affected by nitrogen fertilisation in projected future climatic change environments

BACKGROUNDThe complex interactions between CO2 increase and salinity were investigated in relation to decreased N supply, in order to determine the nutritional quality of broccoli (Brassica oleracea L. var. italica) plants under these conditions. Three different decreased N fertilisation regimes (NO3-/NH4+ ratios of 100:0, 50:50 and 0:100 respectively) were combined with ambient (380ppm) and elevated (800ppm) [CO2] under non-saline (0mmolL(-1) NaCl) and saline (80mmolL(-1) NaCl) conditions. Nutrients (minerals, soluble protein and total amino acids) and natural antioxidants (glucosinolates, phenolic acids, flavonoids and vitamin C) were determined. RESULTSIn NH4+-fed broccoli plants, a marked growth reduction was shown and a redistribution of amino acids to cope with NH4+ toxicity resulted in higher levels of indolic glucosinolate and total phenolic compounds. However, the positive effect of the higher [CO2]-ameliorating adverse effects of salinity-was only observed when N was supplied as NO3-. Under reduced N fertilisation, the total glucosinolates were increased by a decreased NO3-/NH4+ ratio and elevated [CO2] but were unaffected by salinity. CONCLUSIONUnder future climatic challenges, such as increased salinity and elevated [CO2], a clear genotypic dependence of S metabolism was observed in broccoli plants. In addition, an influence of the form in which N was supplied on plant nutritional quality was observed; a combined NO3-/NH4+ (50:50) supply allowed broccoli plants not only to deal with NH4+ toxicity but also to modify their glucosinolate content and profile. Thus, for different modes of N fertilisation, the interaction with climatic factors must be considered in the search for an optimal balance between yield and nutritional quality. (c) 2015 Society of Chemical Industry