Enhanced production of 3-hydroxypropionic acid from glucose via malonyl-CoA pathway by engineered Escherichia coli

被引：43
作者：
Cheng, Zhuan [1 ]
Jiang, Jiaqi [1 ]
Wu, Hui [1 ]
Li, Zhimin [1 ,2 ]
Ye, Qin [1 ]
机构：
[1] E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China
[2] Shanghai Collaborat Innovat Ctr Biomfg Technol, Shanghai 200237, Peoples R China
来源：
BIORESOURCE TECHNOLOGY | 2016年 / 200卷
基金：
美国国家科学基金会; 国家高技术研究发展计划(863计划);
关键词：
3-Hydroxypropionate; Acetyl-CoA carboxylase; Malonyl-CoA reductase; Corynebacterium glutamicum; Escherichia coli; ACETYL-COA; BIOTIN CARBOXYLASE; GENE; EXPRESSION; COENZYME; CLONING; METABOLISM; GLYCEROL; SUBUNIT; CARBON;
D O I：
10.1016/j.biortech.2015.10.107
中图分类号：
S2 [农业工程];
学科分类号：
0828 ;
摘要：
In this study, production of 3-HP via malonyl-CoA was investigated by using metabolically engineered Escherichia coli carrying heterogeneous acetyl-CoA carboxylase (Acc) from Corynebacterium glutamicum and codon-optimized malonyl-CoA reductase (MCR) from Chloroflexus aurantiacus. Three engineered E. coli strains with different host-vector systems were constructed and investigated. The results indicated that the combination of E. coli BL21(DE3) and pET28a was the most efficient host-vector system for 3-HP production, and the highest concentration of 3-HP attained in shake flask cultivation reached 1.80 g/L by the strain BE-MDA with induction at 0.25 mM IPTG and 25 degrees C, and supplementation of NaHCO3 and biotin. In fed-batch fermentation performed in a 5-L reactor, the concentration of 3-HP achieved 10.08 g/L in 36 h. (C) 2015 Elsevier Ltd. All rights reserved.
引用
收藏
页码：897 / 904
页数：8
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