A sensitive DNA biosensor fabricated from gold nanoparticles and graphene oxide on a glassy carbon electrode

被引：52
作者：
Hajihosseini, Saeedeh [1 ]
Nasirizadeh, Navid [2 ]
Hejazi, Mohammad Saeid [3 ]
Yaghmaei, Parichereh [1 ]
机构：
[1] Islamic Azad Univ, Sci & Res Branch, Dept Biol, Tehran, Iran
[2] Islamic Azad Univ, Dept Text & Polymer Engn, Yazd Branch, Yazd, Iran
[3] Tabriz Univ Med Sci, Fac Pharm, Tabriz, Iran
来源：
MATERIALS SCIENCE AND ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS | 2016年 / 61卷
关键词：
DNA biosensor; Helicobacter pylori; Oracet blue; Electrochemical method; HELICOBACTER-PYLORI; MASS-SPECTROMETRY; METHYLENE-BLUE; ACID; NANOCOMPOSITE; NANORODS; SHEETS; SENSOR; FILM;
D O I：
10.1016/j.msec.2015.12.091
中图分类号：
TB3 [工程材料学]; R318.08 [生物材料学];
学科分类号：
0805 ; 080501 ; 080502 ;
摘要：
A sensitive electrochemical DNA biosensor was developed for Helicobacter pylori (H. pylori) detection using differential pulse voltammetry. Single-stranded DNA probe was immobilized on a graphene oxide/gold nanoparticles modified glassy carbon electrode (GO/AuNPs/GCE). A hybridization reaction was conducted with the target DNA and the immobilized DNA on the electrode surface. Oracet blue (OB) was selected for the first time as a redox indicator for amplifying the electrochemical signal of DNA. Enhanced sensitivity was achieved through combining the excellent electric conductivity of GO/AuNPs and the electroactivity of the OB. The DNA biosensor displayed excellent performance to demonstrate the differences between the voltammetric signals of the OB obtained from different hybridization samples (non-complementary, mismatch and complementary DNA5). The proposed biosensor has a linear range of 60.0-600.0 pM and a detection limit of 27.0 pM for detection of H. pylori. In addition, the biosensor have responded very well in the simulated real sample evaluations, signifying its potential to be used in future clinical detection of the H. pylori bacteria. (C) 2015 Elsevier B.V. All rights reserved.
引用
收藏
页码：506 / 515
页数：10
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