Evaluation of antimutagenic activity and mechanisms of action of β-glucan from barley, in CHO-k1 and HTC cell lines using the micronucleus test

被引:59
作者
Oliveira, Rodrigo Juliano
Ribeiro, Lucia Regina
da Silva, Ariane Fernanda
Matuo, Renata
Mantovani, Mario Sergio [1 ]
机构
[1] Univ Estadual Londrina, Dept Biol Geral, Londrina, PR, Brazil
[2] UNESP, Dept Biol Celular, Rio Claro, SP, Brazil
关键词
beta-glucan; HTC cells; CHO-k1; cells; mechanism of action; antimutagenesis; CANCER; APOPTOSIS;
D O I
10.1016/j.tiv.2006.04.001
中图分类号
R99 [毒物学(毒理学)];
学科分类号
100405 ;
摘要
Due to the need to identify new antimutagenic agents and to determine their mechanism of action, the present study examined the mechanism of action of the P-glucan with regard to antimutagenicity using the micronucleus assay in CHO-kl and HTC cell lines. The mutagenicity experiments were performed with three different concentrations of P-glucan (5, 10, and 20 mu g/mL), in wich only the highest dose showed mutagenic activity. In the antimutagenicity experiments, the same concentrations of P-glucan were combined with a mutagenic agent, methylmethane sulfonate, or 2-aminoanthracene, using four different treatment protocols: pre-treatment, simultaneous treatment (simple and with pre-incubation), and post-treatment. The results indicate that the CHO-kl cell line treated with MMS presented a chemopreventive activity for all the doses of P-glucan in the different treatment protocols, except for the lowest dose in post-treatment. When HTC cell line treated with MMS is analysed, a chemopreventive activity can be verified for the highest dose in both pre- and post-treatment. For the simple simultaneous treatment, the three doses demonstrated efficacy, while for the simultaneous treatment with pre-incubation only the intermediate concentration was effective. In HTC treated with 2AA both the lowest dose in the pre-treatment protocol and the post-treatment protocol did not show efficacy in preventing DNA damage. The evaluation of the different protocols and the damage decrease percentages observed suggest that P-glucan has both desmutagenic and bioantimutagenic activity. It is necessary, however, to note that efficacy and mechanism of action are subject to variation when compared the two cell lines, since in HTC, representing a drug-metabolizing system, this substance can show a diminished chemopreventive capacity. (c) 2006 Elsevier Ltd. All rights reserved.
引用
收藏
页码:1225 / 1233
页数:9
相关论文
共 38 条
[1]  
ABDELRAZZAK Z, 1994, MOL PHARMACOL, V46, P1100
[2]  
Borges VC, 2001, NUTR ORAL ENTERAL PA, P1495
[3]  
Bradham CA, 1998, AM J PHYSIOL-GASTR L, V275, pG387, DOI 10.1152/ajpgi.1998.275.3.G387
[4]  
Brusick D., 1987, PRINCIPLES GENETIC T, P284
[5]  
CHORVATOVICOVA D, 1991, Strahlentherapie und Onkologie, V167, P612
[6]   Mechanisms of inhibitors of mutagenesis and carcinogenesis [J].
De Flora, S .
MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS, 1998, 402 (1-2) :151-158
[7]   COMPARATIVE TUMOR-INHIBITORY AND ANTI-BACTERIAL ACTIVITY OF SOLUBLE AND PARTICULATE GLUCAN [J].
DILUZIO, NR ;
WILLIAMS, DL ;
MCNAMEE, RB ;
EDWARDS, BF ;
KITAHAMA, A .
INTERNATIONAL JOURNAL OF CANCER, 1979, 24 (06) :773-779
[8]   The cytokine stimulating activity of (1→3)-β-D-glucans is dependent on the triple helix conformation [J].
Falch, BH ;
Espevik, T ;
Ryan, L ;
Stokke, BT .
CARBOHYDRATE RESEARCH, 2000, 329 (03) :587-596
[9]   Necrosis, apoptosis, cytostasis and DNA damage in human lymphocytes measured simultaneously within the cytokinesis-block micronucleus assay: description of the method and results for hydrogen peroxide [J].
Fenech, M ;
Crott, J ;
Turner, J ;
Brown, S .
MUTAGENESIS, 1999, 14 (06) :605-612
[10]   ANTIMUTAGENS AS CANCER CHEMOPREVENTIVE AGENTS IN THE DIET [J].
FERGUSON, LR .
MUTATION RESEARCH, 1994, 307 (01) :395-410