Assessment of Conjugal Transfer of Antibiotic Resistance Genes in Salmonella Typhimurium Exposed to Bile Salts

被引：6

作者：

He, Xinlong ^{[1
]}

Ahn, Juhee ^{[1
]}

机构：

[1] Kangwon Natl Univ, Dept Med Biomat Engn, Chunchon 200701, South Korea

来源：

JOURNAL OF MICROBIOLOGY | 2014年 / 52卷 / 08期

基金：

新加坡国家研究基金会;

关键词：

Salmonella Typhimurium; antibiotic resistance; virulence; gene transfer; bile salt; ANTIMICROBIAL RESISTANCE; BACTERIAL CONJUGATION; PLASMID; INTEGRONS; CASSETTES; VIRULENCE; EXPRESSION; ANIMALS;

D O I：

10.1007/s12275-014-3340-9

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

This study was designed to evaluate the transfer potential of antibiotic resistance genes in antibiotic-resistant Salmonella Typhimurium (S. Typhimurium(R)) in the presence of bile salts. The resistance of S. Typhimurium(R) to ampicillin, kanamycin, and tetracycline was increased by 64-, 64-, and 512-fold, respectively. The highest transfer frequency from S. Typhimurium(R) to Escherichia colt was observed at the bile salt concentration of 160 mu g/ml (3.8 x 10(-3) transferrants/cells). The expression of traJ and traY was suppressed in S. Typhimurium(R) by bile salt. This study provides useful information for understanding the conjugative transfer of antibiotic resistance genes in S. Typhimurium under intestinal conditions.

引用

页码：716 / 719

页数：4

共 21 条

[1] Bile salt activation of stress response promoters in Escherichia coli [J].

Bernstein, C ;

Bernstein, H ;

Payne, CM ;

Beard, SE ;

Schneider, J .

CURRENT MICROBIOLOGY, 1999, 39 (02) :68-72

[2] Conjugative DNA metabolism in Gram-negative bacteria [J].

de la Cruz, Fernando ;

Frost, Laura S. ;

Meyer, Richard J. ;

Zechner, Ellen L. .

FEMS MICROBIOLOGY REVIEWS, 2010, 34 (01) :18-40

[3] Towards more virulent and antibiotic-resistant Salmonella? [J].

Fluit, AC .

FEMS IMMUNOLOGY AND MEDICAL MICROBIOLOGY, 2005, 43 (01) :1-11

[4] Class 1 integrons, gene cassettes, mobility, and epidemiology [J].

Fluit, AC ;

Schmitz, FJ .

EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES, 1999, 18 (11) :761-770

[5]

Frost LS, 2010, FUTURE MICROBIOL, V5, P1057, DOI [10.2217/fmb.10.70, 10.2217/FMB.10.70]

[6] Conjugal transfer of the Salmonella enterica virulence plasmid in the mouse intestine [J].

Garcia-Quintanilla, Meritxell ;

Ramos-Morales, Francisco ;

Casadesus, Josep .

JOURNAL OF BACTERIOLOGY, 2008, 190 (06) :1922-1927

[7] Conjugative plasmid transfer in gram-positive bacteria [J].

Grohmann, E ;

Muth, G ;

Espinosa, M .

MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS, 2003, 67 (02) :277-+

[8] Characterization of a self-transferable plasmid from Salmonella enterica serotype typhimurium clinical isolates carrying two integron-borne gene cassettes together with virulence and drug resistance genes [J].

Guerra, B ;

Soto, S ;

Helmuth, R ;

Mendoza, MC .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2002, 46 (09) :2977-2981

[9] Characterisation of antimicrobial resistance patterns and class I integrons among Escherichia coli and Salmonella enterica serovar Choleraesuis strains isolated from humans and swine in Taiwan [J].

Hsu, Shu-Chen ;

Chiu, Tsai-Hsin ;

Pang, Jen-Chieh ;

Hsuan-Yuan, Chao-Hsiu ;

Chang, Gan-Nan ;

Tsen, Hau-Yang .

INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS, 2006, 27 (05) :383-391

[10] Antimicrobial resistance in Salmonella isolated from animals and their environment in England and Wales from 1988 to 1999 [J].

Jones, YE ;

Chappell, S ;

McLaren, IM ;

Davies, RH ;

Wray, C .

VETERINARY RECORD, 2002, 150 (21) :649-654

← 1 2 3 →