Sensitive detection of polynucleotide kinase using rolling circle amplification-induced chemiluminescence

被引：37
|
作者：
Tang, Wei [1 ]
Zhu, Guichi [1 ]
Zhang, Chun-Yang [1 ]
机构：
[1] Chinese Acad Sci, Shenzhen Inst Adv Technol, Single Mol Detect & Imaging Lab, Shenzhen 518055, Peoples R China
来源：
CHEMICAL COMMUNICATIONS | 2014年 / 50卷 / 36期
基金：
中国国家自然科学基金;
关键词：
REPAIR; ASSAY; NANOPARTICLE; MECHANISMS; POLYAMINES; MUTATIONS; SEIZURES;
D O I：
10.1039/c4cc00256c
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
We develop a new method for the sensitive detection of polynucleotide kinase (PNK) using rolling circle amplification-induced chemiluminescence. This method exhibits high sensitivity with a detection limit of 2.20 x 10(-4) U mL(-1), which is superior to most reported approaches. Moreover, this method can be used to screen both the inhibitors and the activators of PNK, and can be further applied for real sample analysis.
引用
收藏
页码：4733 / 4735
页数：3
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