MtTdp1α-depleted Medicago truncatula plants show reduced cuticle permeability and altered expression of defense genes

被引：1

作者：

Dona, M. ^{[1
,5
]}

Sabatini, M. E. ^{[1
]}

Biggiogera, M. ^{[1
]}

Confalonieri, M. ^{[2
]}

Minio, A. ^{[3
]}

Delledonne, M. ^{[3
]}

Giraffa, G. ^{[2
]}

Carbonera, D. ^{[1
]}

Araujo, S. ^{[4
,6
]}

Balestrazzi, A. ^{[1
]}

机构：

[1] Dept Biol & Biotechnol L Spallanzani, IT-27100 Pavia, Italy

[2] Ctr Ric Prod Foraggere & Lattiero Casearie, CRA, IT-29600 Lodi, Italy

[3] Univ Verona, Dept Biotechnol, IT-37134 Verona, Italy

[4] ITQB UNL, Plant Cell Biotechnol Lab, P-2780157 Oeiras, Portugal

[5] Austrian Acad Sci, GMI, Vienna Bioctr VBC, A-1030 Vienna, Austria

[6] Dept Biol & Biotechnol L Spallanzani, I-27100 Pavia, Italy

来源：

BIOLOGIA PLANTARUM | 2017年 / 61卷 / 01期

关键词：

cuticular waxes; RNA-Seq; transgenic plants; tyrosyl-DNA phosphodiesterase; TRANSCRIPTION FACTOR; PROTEIN; RESISTANCE; RESPONSES;

D O I：

10.1007/s10535-016-0664-9

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

The link between the MtTdp1 alpha (tyrosyl-DNA phosphodiesterase) gene, involved in the repair of DNA topoisomerase I mediated DNA damage, and the plant defense response has been investigated in MtTdp1 alpha-depleted Medicago truncatula transgenic lines obtained by intron-spliced hairpin RNA approach, compared to the control line (CTRL, empty vector). Reduction of cuticle permeability highlighted by chlorophyll efflux assays positively correlated with the level of MtTdp1 alpha gene silencing. The increased cuticle thickness was confirmed by transmission electron microscopy, which revealed an apparent expansion of the epicuticular waxes deposited on the outer surface. RNA-Seq analysis, carried out in the MtTdp1 alpha-depleted plants, revealed the different expression of resistance (R) genes, PAMP (pathogen-associated-molecular pattern) triggered immunity (PTI) genes and transcription factors (TFs) involved in the regulation of the plant defense response.

引用

页码：192 / 196

页数：5

共 2 条

[1] Depletion of tyrosyl-DNA phosphodiesterase 1α (MtTdp1α) affects transposon expression in Medicago truncatula
Sabatini, Maria Elisa
Dona, Mattia
Leonetti, Paola
Minio, Andrea
Delledonne, Massimo
Carboneral, Daniela
Confalonieri, Massimo
Giraffa, Giorgio
Balestrazzi, Alma
JOURNAL OF INTEGRATIVE PLANT BIOLOGY, 2016, 58 (07) : 618 - 622
[2] Symbiosis-specific expression of two Medicago truncatula nodulin genes, MtN1 and MtN13, encoding products homologous to plant defense proteins
Gamas, P
de Billy, F
Truchet, G
MOLECULAR PLANT-MICROBE INTERACTIONS, 1998, 11 (05) : 393 - 403

← 1 →