Comparison of mesoporous silicate supports for the immobilisation and activity of cytochrome c and lipase

被引：13

作者：

Abdallah, Noreldeen H. ^{[1
]}

Schlumpberger, Miriam ^{[1
]}

Gaffney, Darragh A. ^{[1
]}

Hanrahan, John P. ^{[2
]}

Tobin, Joseph M. ^{[2
]}

Magner, Edmond ^{[1
]}

机构：

[1] Univ Limerick, Dept Chem & Environm Sci, Mat & Surface Sci Inst, Limerick, Ireland

[2] Glantreo Ltd, Cork, Ireland

来源：

JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC | 2014年 / 108卷

基金：

爱尔兰科学基金会;

关键词：

soporous silicate; Immobilisation; Lipase; Cytochrome c; CANDIDA-ANTARCTICA; ADSORPTION; ENZYMES; BIOCATALYST; PARTICLES; PROTEINS; SURFACE; SBA-15; SIZE;

D O I：

10.1016/j.molcatb.2014.06.007

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The activity and stability of Candida antartica lipase B (CALB) and cytochrome c immobilised onto SBA-15 and a porous spherical silicate material (PPS) were examined. The materials possess similar pore diameters but have different morphologies, pore volumes and surface areas. CALB exhibited higher catalytic activity and stability on SBA-15 when compared to PPS, while cytochrome c showed similar catalytic activity on both materials. The activity of CALB immobilised on SBA-15 was retained (95%) after 7 uses, while CALB immobilised on PPS retained only 43% activity. Such changes can be mainly ascribed to the different physical properties (pore volume, surface area and pore shape) of the supports. (C) 2014 Elsevier B.V. All rights reserved.

引用

页码：82 / 88

页数：7

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