NADH oxidation in a microreactor catalysed by ADH immobilised on γ-Fe2O3 nanoparticles

被引：11

作者：

Salic, Anita ^{[1
,2
]}

Pindric, Katarina ^{[2
]}

Podrepsek, Gordana Hojnik ^{[1
]}

Leitgeb, Maja ^{[1
]}

Zelic, Bruno ^{[2
]}

机构：

[1] Univ Maribor, Fac Chem & Chem Engn, SI-2000 Maribor, Slovenia

[2] Univ Zagreb, Fac Chem Engn & Technol, HR-10000 Zagreb, Croatia

来源：

GREEN PROCESSING AND SYNTHESIS | 2013年 / 2卷 / 06期

关键词：

alcohol dehydrogenase; coenzyme regeneration; enzyme immobilisation; microreactor; maghemite nanoparticle; ALCOHOL-DEHYDROGENASE; NAD(+) REGENERATION; HEXANOL OXIDATION; SUPERPARAMAGNETIC NANOPARTICLES; MAGNETIC NANOPARTICLES; COENZYME REGENERATION; LIPOXYGENASE; MAGHEMITE; BINDING;

D O I：

10.1515/gps-2013-0084

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A new concept of nicotinamide adenine dinucleotide hydrate (NADH) oxidation which combines advantages of the microreactor technology with the advantages of magnetic nanoparticles (MNPs) application is developed. Acetaldehyde was used as a substrate for the NADH regeneration process while the reaction was performed in a batch reactor and in a microreactor using alcohol dehydrogenase (ADH)-loaded MNPs. Three different microreactor systems with MNPs were studied, two with stationary MNPs trapped on the inner surface of microchannel by permanent magnetic field and one where the MNPs actively moved across the channel (movement inside microchannel allowed by an oscillating magnetic field). In a reactor system with an oscillating magnetic field and an actively moving ADH-loaded MNPs 100% NADH conversion was achieved for residence time of just 2 min.

引用

页码：569 / 578

页数：10

共 37 条

[1] Brunerie P., 1997, US Patent, Patent No. [US5620879, 5620879]
[2] REGENERATION OF NICOTINAMIDE COFACTORS FOR USE IN ORGANIC-SYNTHESIS
CHENAULT, HK
WHITESIDES, GM
[J]. APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 1987, 14 (02) : 147 - 197
[3] Application of tris(hydroxymethyl)phosphine as a coupling agent for alcohol dehydrogenase immobilization
Cochrane, FC
Petach, HH
Henderson, W
[J]. ENZYME AND MICROBIAL TECHNOLOGY, 1996, 18 (05) : 373 - 378
[4] Magnetic nanoparticles and their applications in medicine
Duguet, Etienne
Vasseur, Sebastien
Mornet, Stephane
Devoisselle, Jean-Marie
[J]. NANOMEDICINE, 2006, 1 (02) : 157 - 168
[5] Ehrfeld W., 2000, Microreactors: new technology for modern chemistry, DOI DOI 10.1002/3527601953
[6] GODBOLE SS, 1983, ENZYME MICROB TECH, V5, P125, DOI 10.1016/0141-0229(83)90046-7
[7] Novel immobilization routes for the covalent binding of an alcohol dehydrogenase from Rhodococcus ruber DSM 44541
Goldberg, Katja
Krueger, Anke
Meinhardt, Thomas
Kroutil, Wolfgang
Mautner, Barbara
Liese, Andreas
[J]. TETRAHEDRON-ASYMMETRY, 2008, 19 (10) : 1171 - 1173
[8] Characterisation of residence time and residence time distribution in chip reactors with modular arrangements by integrated optical detection
Günther, M
Schneider, S
Wagner, J
Gorges, R
Henkel, T
Kielpinski, M
Albert, J
Bierbaum, R
Köhler, JM
[J]. CHEMICAL ENGINEERING JOURNAL, 2004, 101 (1-3) : 373 - 378
[9] Kang SC, 2007, J NANOSCI NANOTECHNO, V7, P3706, DOI 10.1166/jnn.2007.022
[10] Magnetic force-based multiplexed immunoassay using superparamagnetic nanoparticles in microfluidic channel
Kim, KS
Park, JK
[J]. LAB ON A CHIP, 2005, 5 (06) : 657 - 664

← 1 2 3 4 →