An accurate method of copper and chromium determination in red wine was studied by flame atomic absorption spectrometry. This experiment compared four methods of direct digestion-standard concentration method (DDSC), microwave digestion-standard concentration method (MDSC), direct digestion-standard addition method (DDSA), and microwave digestion-standard addition method (MDSA) according to recovery rates and precision. It was found that the recovery rates of copper and chromium, measured by the microwave digestion-standard addition method were, respectively, 100.85%, 101.07%, and the precision was 1.23%, 0.05%; on the other hand, this method had the better digestion, the fewer elements loss, less interference, the better accuracy and stability. It indicates that the microwave digestion-standard addition method is applicable to red wine for determination of copper and chromium.