Brain neurosteroids during the mouse oestrous cycle

被引:101
作者
Corpechot, C
Collins, BE
Carey, MP
Tsouros, A
Robel, P
Fry, JP
机构
[1] UNIV LONDON UNIV COLL, DEPT PHYSIOL, LONDON WC1E 6BT, ENGLAND
[2] INSERM, U33, F-94276 LE KREMLIN BICETRE, FRANCE
[3] CHURCHILL CLIN, LONDON SE1 7PP, ENGLAND
基金
英国医学研究理事会;
关键词
allopregnanolone; brain; circadian rhythm; mouse; neurosteroid; estrous cycle; 5 alpha-pregnenolone-3,20-dione; pregnenolone sulfate; progesterone;
D O I
10.1016/S0006-8993(97)00749-X
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Concentrations of the neuroactive steroid 3 alpha,5 alpha-tetrahydroprogesterone (TH PROG or allopregnanolone) and its precursors progesterone (PROG) and 5 alpha-dihydroprogesterone (DH PROG) have been measured in mouse brain throughout the oestrous cycle. Plasma PROG concentrations were also measured for comparison. At each stage, circadian fluctuations were found in the concentrations of brain PROG and its metabolites. Such fluctuations were greater than those attributable to any particular stage of the oestrous cycle. Over the entire cycle, a significant correlation was found between brain TH PROG (or DH PROG) and PROG concentrations but not between brain TH PROG (or DH PROG) and plasma PROG concentrations. There was also no correlation between endogenous TH PROG (or DH PROG) and activity of the 5 alpha-reductase converting H-3-PROG to H-3-DH FROG in whole brain homogenates. Concentrations of another neuroactive steroid, pregnenolone sulphate (PREG S), in the brain during the oestrous cycle were in phase with plasma PROG but not brain PROG concentrations. Our results indicate that circadian and ovarian influences on the concentrations of PROG and its metabolite TH PROG in female whole mouse brain are caused predominantly by changes in the supply of PROG from within the tissue, whatever the contribution of peripheral sources. (C) 1997 Elsevier Science B.V.
引用
收藏
页码:276 / 280
页数:5
相关论文
共 40 条
[1]   RODENT SEX-DIFFERENCES IN EMOTIONAL AND RELATED BEHAVIOR [J].
ARCHER, J .
BEHAVIORAL BIOLOGY, 1975, 14 (04) :451-479
[2]  
BACKSTROM T, 1976, ACTA NEUROL SCAND, V54, P321
[3]   DIURNAL PROGESTERONE RHYTHMS IN THE FEMALE MOUSE [J].
BAILEY, KJ .
JOURNAL OF ENDOCRINOLOGY, 1987, 112 (01) :15-21
[4]   NEUROSTEROIDS - A NEW BRAIN-FUNCTION [J].
BAULIEU, EE ;
ROBEL, P .
JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY, 1990, 37 (03) :395-403
[5]   DISTRIBUTION AND OVARIAN CONTROL OF PROGESTIN-METABOLIZING ENZYMES IN VARIOUS RAT HYPOTHALAMIC REGIONS [J].
BERTICS, SJ ;
BERTICS, PJ ;
CLARKE, JL ;
KARAVOLAS, HJ .
JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY, 1987, 26 (03) :321-328
[6]   OVARIAN ENDOCRINE STATUS MODULATES THE ANXIOLYTIC POTENCY OF DIAZEPAM AND THE EFFICACY OF GAMMA-AMINOBUTYRIC-ACID BENZODIAZEPINE RECEPTOR MEDIATED CHLORIDE-ION TRANSPORT [J].
BITRAN, D ;
HILVERS, RJ ;
KELLOGG, CK .
BEHAVIORAL NEUROSCIENCE, 1991, 105 (05) :653-662
[7]  
BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[8]   EMERGING DIVERSITIES IN THE MECHANISM OF ACTION OF STEROID-HORMONES [J].
BRANN, DW ;
HENDRY, LB ;
MAHESH, VB .
JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY, 1995, 52 (02) :113-133
[9]   PLASMA CONCENTRATION OF LH, FSH, PROLACTIN, PROGESTERONE AND ESTRADIOL-17BETA THROUGHOUT 4-DAY ESTROUS-CYCLE OF RAT [J].
BUTCHER, RL ;
COLLINS, WE ;
FUGO, NW .
ENDOCRINOLOGY, 1974, 94 (06) :1704-1708
[10]   FLUCTUATIONS IN RESPONSES TO DIAZEPAM DURING THE ESTROUS-CYCLE IN THE MOUSE [J].
CAREY, MP ;
BILLING, AE ;
FRY, JP .
PHARMACOLOGY BIOCHEMISTRY AND BEHAVIOR, 1992, 41 (04) :719-725