In this study conducted in order to evaluate the differences between some chemical properties of chestnut fruits(Castanea sativa Mill.) picked from 4 different regions as Bartin-Kumluca, Bartin-Karacaydere, Devrek-Akcasu and caycuma-Filyos and to find the information shedding light to consumers and producers about the practice of usage of chestnut fruit; it has been determined that the moisture content of chestnut fruits have been found to vary between 50.89 and 58.49, and the Kumluca origin has taken the first place in terms of moisture content. Another chemical property investigated in this study is the crude protein. The mean crude protein values determined in chestnut fruits vary between 5.59 and 7.29. As a result of performed Duncan test results, the Kumluca origin took place in first group in terms of crude material content. The crude oil content varies between 2.7% and 4.6% among the origins. According to the result of Duncan test, Akcasu origin takes the first place in terms of crude oil content. In this study, the ash contents of chestnut fruits have also been investigated. Accordingly, ash content was varying between 2.29 and 2.99, and the highest amount of ash content has been found in Filyos origin. While calculating the nutritional value, the total carbohydrate content of that nufrient should be known too. In this context, the carbohydrate contents of 4 chestnut fruit origins picked have been determined. Accordingly, the total carbohydrate content of fruit origins were varying between 78.49 and 93.39. As a result of performed Duncan test, the Kumluca origin takes the first place in terms of total carbohydrate content. Determined via ICP-AES device, the total phenolic content varies between 137.8mg/100g and 386.4mg/100g among the origins. As a result of Duncan test, the Kumluca and Karacaydere origins took the first place in terms of total phenolic content, followed by Akcasu and Filyos origins. Within the scope of this study, also the mineral contents of 4 chestnut fruit origins picked have been investigated. Accordingly, it has been determined that the Ca amount varies between 2040mg/kg and 2937mg/kg among the origins. And according to Duncan test, Filyos and Akcasu origins take the first place in terms of Ca content. Mg varies between 1364mg/kg and 1852mg/kg among the origins, and Kumluca origin takes the first place according to Duncan test results. While Na varies between 245mg/kg and 526mg/kg among the origins, Kumluca origin takes the first place in terms of Na content according to the results of Duncan test. It has been determined that K content of origins varies between 10633mg/kg and 15871mg/kg. According to Duncan test, chestnut fruits of Akcasu origin take the first place in terms of K content. As a result of chemical analyses within the scope of this study, it has been determined that the P content of the origins varies between 1741mg/kg and 1879mg/kg. According to Duncan test, Akcasu and Filyos origins take the first place in terms of P content. Within the scope of this research, also the Zn content of chestnut fruits of picked origins has been investigated too. As a result of performed chemical analyses, it has been determined that the Zn contents of origins picked vary between 54mg/kg and 85mg/kg, while Filyos origin takes place in first group in terms of Zn content according to Duncan test results.