Inhibition of quorum sensing, biofilm, and spoilage potential in Shewanella baltica by green tea polyphenols

被引：70
作者：
Zhu, Junli [1 ]
Huang, Xuzheng [1 ]
Zhang, Fang [1 ]
Feng, Lifang [1 ]
Li, Jianrong [2 ]
机构：
[1] Zhejiung Gongshang Univ, Coll Food Sci & Biotechnol, Hangzhou 310018, Zhejiang, Peoples R China
[2] Bohai Univ, Coll Chem Chem Engn & Food Safety, Food Safety Key Lab Liaoning Prov, Jinzhou 121013, Peoples R China
来源：
JOURNAL OF MICROBIOLOGY | 2015年 / 53卷 / 12期
基金：
中国国家自然科学基金;
关键词：
Shewanella baltica; AI-2; diketopiperazines; green tea polyphenols; biofilm; fish spoilage; BACTERIA; PHYTOCHEMICALS; IDENTIFICATION; EXTRACT; STORAGE;
D O I：
10.1007/s12275-015-5123-3
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
We investigated the quorum sensing (QS) system of Shewanella baltica and the anti-QS related activities of green tea polyphenols (TP) against spoilage bacteria in refrigerated large yellow croaker. Autoinducer-2 (AI-2) and the diketopiperazines (DKPs) cyclo-(L-Pro-L-Leu) and cyclo- (L-Pro-L-Phe) were detected in the culture extract of S. baltica XH2, however, no N-acylhomoserine lactones (AHLs) activity was observed. Green TP at sub-inhibitory concentrations interfered with AI-2 and DKPs activities of S. baltica without inhibiting cell growth and promoted degradation of AI-2. The green TP treatment inhibited biofilm development, exopolysaccharide production and swimming motility of S. baltica in a concentration-dependent manner. In addition, green TP decreased extracellular protease activities and trimethylamine production in S. baltica. A transcriptional analysis showed that green TP repressed the luxS and torA genes in S. baltica, which agreed with the observed reductions in QS activity and the spoilage phenotype. Epigallocatechin gallate (EGCG)-enriched in green TP significantly inhibited AI-2 activity of S.baltica. These findings strongly suggest that green TP could be developed as a new QS inhibitor for seafood preservation to enhance shelf life.
引用
收藏
页码：829 / 836
页数：8
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