Fluorescence quenching effect of guanine interacting with water-soluble cationic porphyrin

被引：33

作者：

Makarska-Bialokoz, Magdalena ^{[1
]}

机构：

[1] Marie Curie Sklodowska Univ, Dept Inorgan Chem, PL-20031 Lublin, Poland

来源：

JOURNAL OF LUMINESCENCE | 2014年 / 147卷

关键词：

Guanine; Water-soluble cationic porphyrin; Fluorescence quenching; Modified Stern-Volmer plot; METAL-COMPLEXES; ELECTRON-TRANSFER; DNA; PHOTOCHEMISTRY; BINDING; AGGREGATION; CAFFEINE; THERAPY; STATE;

D O I：

10.1016/j.jlumin.2013.10.061

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

The process of association between 5,10,15,20-tetrakis[4-(trimethylammonio)phenyl]-21H,23H-porphine tetra-p-tosylate (H2TTMePP) and guanine has been studied both in NaOH solution and TRIS buffer analysing its absorption and steady-state fluorescence spectra. The fluorescence quenching effect observed during interactions porphyrin-guanine points at the fractional accessibility of the fluorophore for the quencher. The association and fluorescence quenching constants are of the order of magnitude of 10(5) mol(-1). The fluorescence lifetimes and the quantum yields of the porphyrin monoanion form were established. The results demonstrate that guanine can interact with H2TTMePP at basic pH and through formation of stacking complexes is able to quench its ability to emission. (C) 2013 Elsevier B.V. All rights reserved.

引用

页码：27 / 33

页数：7

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