Optimization of ultrasonic-assisted extraction (UAE) of phenolics and antioxidant compounds from rhizomes of Rheum moorcroftianum using response surface methodology (RSM)

被引：136
作者：
Pandey, Aseesh [1 ,2 ]
Belwal, Tarun [1 ]
Sekar, K. Chandra [1 ]
Bhatt, Indra D. [1 ]
Rawal, Ranbeer S. [1 ]
机构：
[1] GB Pant Natl Inst Himalayan Environm & Sustainabl, Almora 263643, Uttarakhand, India
[2] GB Pant Natl Inst Himalayan Environm & Sustainabl, Sikkim Unit, Gangtok 737101, Sikkim, India
来源：
INDUSTRIAL CROPS AND PRODUCTS | 2018年 / 119卷
关键词：
Antioxidants; Extraction optimization; Himalaya; Polyphenols; Rheum; UAE; FOOD; POLYSACCHARIDES; DEGRADATION; OXIDATION; PRESSURE; DESIGN;
D O I：
10.1016/j.indcrop.2018.04.019
中图分类号：
S2 [农业工程];
学科分类号：
0828 ;
摘要：
This study for the first time designed to optimize the extraction of polyphenols compounds from rhizomes of Rheum moorcroftianum using response surface methodology (RSM). Solvent was selected based on the preliminary experiments, and a four-factors-three-level, Box-Behnken Design (BBD) including 29 experimental runs. The polyphenols content and antioxidant activity was significantly (p < 0.05) affected by vessel diameter - 6 cm (X-1), sample to solvent ratio - 1:28.42 g/mL (X-2) and extraction temperature - 37.11 degrees C (X-3) under ultrasonic assisted extraction (UAE). The measured parameters were found in accordance with the predicted values. High Performance Liquid Chromatography (HPLC) analysis in optimized condition revealed the presence of 12 phenolic antioxidant compounds with the highest concentration of chlorogenic acid (26.68 mg/g). The results indicate that optimization of extraction conditions in R. moorcroftianum is critical for precise quantification of antioxidant phenolics and its further utilization in industry.
引用
收藏
页码：218 / 225
页数：8
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