In vitro apoptotic effect of cassaine-type diterpene amides from Erythrophleum fordii on PC-3 prostate cancer cells

被引:13
作者
Tran Manh Hung [1 ]
To Dao Cuong [1 ]
Kim, Jeong Ah [2 ]
Lee, Jeong Hyung [3 ]
Woo, Mi Hee [1 ]
Min, Byung Sun [1 ]
机构
[1] Catholic Univ Daegu, Coll Pharm, Gyeongbuk 712702, South Korea
[2] Kyungpook Natl Univ, Pharmaceut Sci Res Inst, Coll Pharm, Taegu 702701, South Korea
[3] Kangwon Natl Univ, Coll Nat Sci, Kangwon 200701, South Korea
基金
新加坡国家研究基金会;
关键词
Erythrophleum fordii; Leguminosae; Cassaine diterpenoid amide; Erythrophlesin H; PC-3; apoptosis;
D O I
10.1016/j.bmcl.2014.09.025
中图分类号
R914 [药物化学];
学科分类号
100701 ;
摘要
Cytotoxic activity-guided fractionation of Erythrophleum fordii led to the isolation of two new cassaine diterpenoid-diterpenoid amide dimers, erythrophlesins H-I (1, 2). Spectral data indicated that they consist of asymmetrical dimeric structure via an ester bond between two cassaine diterpenoids. MTT assay confirmed that compound 1, erythrophlesin H, had the strongest cytotoxic effect toward the human prostate cancer cell line, PC-3. The molecular mechanism by which this compound induced apoptosis cell in prostate cancer remains unknown. Erythrophlesin H induced apoptosis in a dose-dependent manner. Acridine orange and annexin V-FITC/PI double staining confirmed that erythrophlesin H effectively induces apoptosis in PC-3 cells. (C) 2014 Elsevier Ltd. All rights reserved.
引用
收藏
页码:4989 / 4994
页数:6
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