In Vitro and In Vivo Plant Growth Promoting Activities and DNA Fingerprinting of Antagonistic Endophytic Actinomycetes Associates with Medicinal Plants

被引:103
作者
Passari, Ajit Kumar [1 ]
Mishra, Vineet Kumar [1 ]
Gupta, Vijai Kumar [2 ]
Yadav, Mukesh Kumar [3 ]
Saikia, Ratul [4 ]
Singh, Bhim Pratap [1 ]
机构
[1] Mizoram Univ, Dept Biotechnol, Mol Microbiol & Systemat Lab, Mizoram 796004, India
[2] Natl Univ Ireland Galway, Dept Biochem, Mol Glyco Biotechnol Grp, Galway, Ireland
[3] Korea Univ, Coll Med, Seoul 136705, South Korea
[4] CSIR North East Inst Sci & Technol, Div Biotechnol, Jorhat 785006, Assam, India
关键词
ANTIMICROBIAL ACTIVITY; MICROBIAL DIVERSITY; DIFFERENT CULTIVARS; WILT PATHOGEN; STREPTOMYCES; SOIL; CHITINASE; BACTERIA; ECOLOGY; TOMATO;
D O I
10.1371/journal.pone.0139468
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Endophytic actinomycetes have shown unique plant growth promoting as well as antagonistic activity against fungal phytopathogens. In the present study forty-two endophytic actinomycetes recovered from medicinal plants were evaluated for their antagonistic potential and plant growth-promoting abilities. Twenty-two isolates which showed the inhibitory activity against at least one pathogen were subsequently tested for their plant-growth promoting activities and were compared genotypically using DNA based fingerprinting, including enterobacterial repetitive intergenic consensus (ERIC) and BOX repetitive elements. Genetic relatedness based on both ERIC and BOX-PCR generates specific patterns corresponding to particular genotypes. Exponentially grown antagonistic isolates were used to evaluate phosphate solubilization, siderophores, HCN, ammonia, chitinase, indole-3-acetic acid production, as well as antifungal activities. Out of 22 isolates, the amount of indole-3-acetic acid (IAA) ranging between 10-32 mu g/ml was produced by 20 isolates and all isolates were positive for ammonia production ranging between 5.2 to 54 mg/ml. Among 22 isolates tested, the amount of hydroxamate-type siderophores were produced by 16 isolates ranging between 5.2 to 36.4 mu g/ml, while catechols-type siderophores produced by 5 isolates ranging from 3.2 to 5.4 mu g/ml. Fourteen isolates showed the solubilisation of inorganic phosphorous ranging from 3.2 to 32.6 mg/100ml. Chitinase and HCN production was shown by 19 and 15 different isolates, respectively. In addition, genes of indole acetic acid (iaaM) and chitinase (chiC) were successively amplified from 20 and 19 isolates respectively. The two potential strains Streptomyces sp. (BPSAC34) and Leifsonia xyli (BPSAC24) were tested in vivo and improved a range of growth parameters in chilli (Capsicum annuum L.) under greenhouse conditions. This study is the first published report that actinomycetes can be isolated as endophytes from within these plants and were shown to have antagonistic and plant growth promoting abilities. These results clearly suggest the possibility of using endophytic actinomycetes as bioinoculant for plant growth promotion, nutrient mobilization or as biocontrol agent against fungal phytopathogens for sustainable agriculture.
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页数:18
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