Nucleotide-dependent conformational changes in the DnaA-like core of the origin recognition complex

被引：72
作者：
Clarey, Megan G.
Erzberger, Jan P.
Grob, Patricia
Leschziner, Andres E.
Berger, James M.
Nogales, Eva
Botchan, Michael
机构：
[1] Univ Calif Berkeley, Div Biochem & Mol Biol, Dept Mol & Cellular Biochem, Berkeley, CA 94720 USA
[2] Lawrence Berkeley Natl Lab, Berkeley, CA 94720 USA
[3] Univ Calif Berkeley, Howard Hughes Med Inst, Dept Cell & Mol Biol, Berkeley, CA 94720 USA
来源：
NATURE STRUCTURAL & MOLECULAR BIOLOGY | 2006年 / 13卷 / 08期
关键词：
D O I：
10.1038/nsmb1121
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Structural details of initiator proteins for DNA replication have provided clues to the molecular events in this process. EM reconstructions of the Drosophila melanogaster origin recognition complex (ORC) reveal nucleotide-dependent conformational changes in the core of the complex. All five AAA+ domains in ORC contain a conserved structural element that, in DnaA, promotes formation of a right-handed helix, indicating that helical AAA+ substructures may be a feature of all initiators. A DnaA helical pentamer can be docked into ORC, and the location of Orc5 uniquely positions this core. The results suggest that ATP-dependent conformational changes observed in ORC derive from reorientation of the AAA+ domains. By analogy to the DNA-wrapping activity of DnaA, we posit that ORC together with Cdc6 prepares origin DNA for helicase loading through mechanisms related to the established pathway of prokaryotes.
引用
收藏
页码：684 / 690
页数：7
相关论文
共 50 条
[1]   TRANSCRIPTIONAL ACTIVATION OF INITIATION OF REPLICATION FROM THE ESCHERICHIA-COLI CHROMOSOMAL ORIGIN - AN RNA-DNA HYBRID NEAR ORIC [J].
BAKER, TA ;
KORNBERG, A .
CELL, 1988, 55 (01) :113-123
[2]   The origin recognition complex: from simple origins to complex functions [J].
Bell, SP .
GENES & DEVELOPMENT, 2002, 16 (06) :659-672
[3]   ATP-DEPENDENT RECOGNITION OF EUKARYOTIC ORIGINS OF DNA-REPLICATION BY A MULTIPROTEIN COMPLEX [J].
BELL, SP ;
STILLMAN, B .
NATURE, 1992, 357 (6374) :128-134
[4]   ATP hydrolysis by ORC catalyzes reiterative Mcm2-7 assembly at a defined origin of replication [J].
Bowers, JL ;
Randell, JCW ;
Chen, SY ;
Bell, SP .
MOLECULAR CELL, 2004, 16 (06) :967-978
[5]   Structural analysis of a eukaryotic sliding DNA clamp-clamp loader complex [J].
Bowman, GD ;
O'Donnell, M ;
Kuriyan, J .
NATURE, 2004, 429 (6993) :724-730
[6]   A MODEL FOR INITIATION AT ORIGINS OF DNA-REPLICATION [J].
BRAMHILL, D ;
KORNBERG, A .
CELL, 1988, 54 (07) :915-918
[7]   DUPLEX OPENING BY DNAA PROTEIN AT NOVEL SEQUENCES IN INITIATION OF REPLICATION AT THE ORIGIN OF THE ESCHERICHIA-COLI CHROMOSOME [J].
BRAMHILL, D ;
KORNBERG, A .
CELL, 1988, 52 (05) :743-755
[8]   Biochemical characterization of Cdc6/Orc1 binding to the replication origin of the euryarchaeon Methanothermobacter thermoautotrophicus [J].
Capaldi, SA ;
Berger, JM .
NUCLEIC ACIDS RESEARCH, 2004, 32 (16) :4821-4832
[9]   Assembly of functionally active Drosophila origin recognition complex from recombinant proteins [J].
Chesnokov, I ;
Gossen, M ;
Remus, D ;
Botchan, M .
GENES & DEVELOPMENT, 1999, 13 (10) :1289-1296
[10]   Functional analysis of mutant and wild-type Drosophila origin recognition complex [J].
Chesnokov, I ;
Remus, D ;
Botchan, M .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2001, 98 (21) :11997-12002
12345