UCP2 and UCP3 rise in starved rat skeletal muscle but mitochondrial proton conductance is unchanged

被引：209
作者：
Cadenas, S
Buckingham, JA
Samec, S
Seydoux, J
Din, N
Dulloo, AG
Brand, MD
机构：
[1] Univ Cambridge, Dept Biochem, Cambridge CB2 1QW, England
[2] MRC, Dunn Human Nutr Unit, Wellcome Trust, Cambridge CB2 2XY, England
[3] Univ Geneva, Fac Med, Dept Physiol, CH-1211 Geneva 4, Switzerland
[4] Novo Nordisk AS, Dept Mol Genet, DK-2880 Bagsvaerd, Denmark
[5] Univ Fribourg, Inst Physiol, CH-1700 Fribourg, Switzerland
来源：
FEBS LETTERS | 1999年 / 462卷 / 03期
关键词：
uncoupling protein; UCP2; UCP3; fasting; proton leak; skeletal muscle;
D O I：
10.1016/S0014-5793(99)01540-9
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The relationship between UCP2 and UCP3 expression and mitochondrial proton conductance of rat skeletal muscle was examined. Rats were starved for 24 h and the levels of UCP2 and UCP3 mRNA and UCP3 protein mere determined by Northern and Western blots. Proton conductance was measured by titrating mitochondrial respiration rate and membrane potential with malonate, Starvation increased UCP2 and UCP3 mRNA levels more than 5-fold and 4-fold, respectively, and UCP3 protein levels by 2-fold. However, proton conductance remained unchanged. These results suggest either that Northern and Western blots do not reflect the levels of active protein or that these UCPs do not catalyse the basal proton conductance in skeletal muscle mitochondria, (C) 1999 Federation of European Biochemical Societies.
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收藏
页码：257 / 260
页数：4
相关论文
共 27 条
[1]   Tissue-dependent upregulation of rat uncoupling protein-2 expression in response to fasting or cold [J].
Boss, O ;
Samec, S ;
Dulloo, A ;
Seydoux, J ;
Muzzin, P ;
Giacobino, JP .
FEBS LETTERS, 1997, 412 (01) :111-114
[2]   Uncoupling protein-3: A new member of the mitochondrial carrier family with tissue-specific expression [J].
Boss, O ;
Samec, S ;
PaoloniGiacobino, A ;
Rossier, C ;
Dulloo, A ;
Seydoux, J ;
Muzzin, P ;
Giacobino, JP .
FEBS LETTERS, 1997, 408 (01) :39-42
[3]   Uncoupling protein-3 expression in rodent skeletal muscle is modulated by food intake but not by changes in environmental temperature [J].
Boss, O ;
Samec, S ;
Kühne, F ;
Bijlenga, P ;
Assimacopoulos-Jeannet, F ;
Seydoux, J ;
Giacobino, JP ;
Muzzin, P .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (01) :5-8
[4]   THE CAUSES AND FUNCTIONS OF MITOCHONDRIAL PROTON LEAK [J].
BRAND, MD ;
CHIEN, LF ;
AINSCOW, EK ;
ROLFE, DFS ;
PORTER, RK .
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS, 1994, 1187 (02) :132-139
[5]  
BRAND MD, 1999, INT J OBESITY, V23, P1
[6]  
Cannon B, 1999, PROG OBES R, P13
[7]   BIOCHEMICAL AND OSMOTIC PROPERTIES OF SKELETAL MUSCLE MITOCHONDRIA [J].
CHAPPELL, JB ;
PERRY, SV .
NATURE, 1954, 173 (4414) :1094-1095
[8]   Uncoupling protein-2: A novel gene linked to obesity and hyperinsulinemia [J].
Fleury, C ;
Neverova, M ;
Collins, S ;
Raimbault, S ;
Champigny, O ;
LeviMeyrueis, C ;
Bouillaud, F ;
Seldin, MF ;
Surwit, RS ;
Ricquier, D ;
Warden, CH .
NATURE GENETICS, 1997, 15 (03) :269-272
[9]   Cloning and characterization of an uncoupling protein homolog - A potential molecular mediator of human thermogenesis [J].
Gimeno, RE ;
Dembski, M ;
Weng, X ;
Deng, NH ;
Shyjan, AW ;
Gimeno, CJ ;
Iris, F ;
Ellis, SJ ;
Woolf, EA ;
Tartaglia, LA .
DIABETES, 1997, 46 (05) :900-906
[10]   Uncoupling protein-3 is a mediator of thermogenesis regulated by thyroid hormone, beta 3-adrenergic agonists, and leptin [J].
Gong, DW ;
He, YF ;
Karas, M ;
Reitman, M .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (39) :24129-24132
123