Construction of arginine-rich peptide displaying bionanocapsules

被引：16

作者：

Shishido, Takuya ^{[2
]}

Yonezawa, Daisaku ^{[3
]}

Iwata, Kiyokazu ^{[3
]}

Tanaka, Tsutomu ^{[4
]}

Ogino, Chiaki ^{[1
]}

Fukuda, Hideki ^{[4
]}

Kondo, Akihiko ^{[1
]}

机构：

[1] Kobe Univ, Grad Sch Engn, Dept Chem Sci & Engn, Nada Ku, Kobe, Hyogo 6578501, Japan

[2] Kobe Univ, Grad Sch Sci & Technol, Dept Mol Sci & Mat Engn, Nada Ku, Kobe, Hyogo 6578501, Japan

[3] Kobe Univ, Grad Sch Sci & Technol, Dept Chem Sci & Engn, Nada Ku, Kobe, Hyogo 6578501, Japan

[4] Kobe Univ, Org Adv Sci & Technol, Nada Ku, Kobe, Hyogo 6578501, Japan

来源：

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 2009年 / 19卷 / 05期

关键词：

Drug delivery; Bionanocapsule; Cell-penetrating peptide; Octaarginine; Tat; Antennapedia; INTRACELLULAR DELIVERY; CELLULAR-UPTAKE; PROTEIN; PARTICLES; MECHANISMS; LIPOSOMES; MEMBRANE; CELLS;

D O I：

10.1016/j.bmcl.2009.01.049

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

Bionanocapsule (BNC) is a hollow nanoparticle composed of L-protein of the hepatitis B virus surface antigen. BNC can deliver genes or drugs into specific human hepatocytes, but delivery is limited to hepatocytes. In this study, we attempted to alter the specificity of BNCs by genetically introducing cell-penetrating peptides (CPPs), such as arginine-rich peptides, into BNCs. The CPP-fused BNC was efficiently internalized into various cell lines in a short period without significant cytotoxicity. These results show that CPP-BNC could be applied as an efficient carrier for gene and drug delivery. (C) 2009 Elsevier Ltd. All rights reserved.

引用

页码：1473 / 1476

页数：4

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