Human in vivo radiation-induced biomarkers:: Gene expression changes in radiotherapy patients

被引：196
作者：
Amundson, SA
Grace, MB
McLeland, CB
Epperly, MW
Yeager, A
Zhan, QM
Greenberger, JS
Fornace, AJ
机构：
[1] NIH, Ctr Canc Res, NCI, Bethesda, MD USA
[2] Armed Forces Radiobiol Res Inst, Biol Dosimetry Team, Bethesda, MD USA
[3] Univ Pittsburgh, Inst Canc, Pittsburgh, PA USA
来源：
CANCER RESEARCH | 2004年 / 64卷 / 18期
关键词：
D O I：
10.1158/0008-5472.CAN-04-1883
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
After initially identifying potential biomarkers of radiation exposure through microarray studies of ex vivo irradiated human peripheral white blood cells, we have now measured the in vivo responses of several of these biomarker genes in patients undergoing total body irradiation. Microarray analysis has identified additional in vivo radiation-responsive genes, although the general in vivo patterns of stress-gene induction appear similar to those obtained from ex vivo white blood cell experiments. Additional studies may reveal correlations between responses and either diagnosis or prognosis, and such in vivo validation marks an important step in the development of potentially informative radiation exposure biomarkers.
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收藏
页码：6368 / 6371
页数：4
相关论文
共 16 条
[1]  
Amundson SA, 2000, RADIAT RES, V154, P342, DOI 10.1667/0033-7587(2000)154[0342:IOPMBI]2.0.CO
[2]  
2
[3]   Fluorescent cDNA microarray hybridization reveals complexity and heterogeneity of cellular genotoxic stress responses [J].
Amundson, SA ;
Bittner, M ;
Chen, YD ;
Trent, J ;
Meltzer, P ;
Fornace, AJ .
ONCOGENE, 1999, 18 (24) :3666-3672
[4]  
AMUNDSON SA, 2001, EXPERT REV MOL DIAGN, V1, P89
[5]  
Bae I, 1996, CANCER RES, V56, P840
[6]  
Blakely WF, 2001, RADIAT PROT DOSIM, V97, P17, DOI 10.1093/oxfordjournals.rpd.a006633
[7]  
Chen Y, 1997, J Biomed Opt, V2, P364, DOI 10.1117/12.281504
[8]   Ratio statistics of gene expression levels and applications to microarray data analysis [J].
Chen, YD ;
Kamat, V ;
Dougherty, ER ;
Bittner, ML ;
Meltzer, PS ;
Trent, JM .
BIOINFORMATICS, 2002, 18 (09) :1207-1215
[9]  
DeRisi J, 1996, NAT GENET, V14, P457
[10]   Development and assessment of a quantitative reverse transcription-PCR assay for simultaneous measurement of four amplicons [J].
Grace, MB ;
McLeland, CB ;
Gagliardi, SJ ;
Smith, JM ;
Jackson, WE ;
Blakely, WF .
CLINICAL CHEMISTRY, 2003, 49 (09) :1467-1475
12