A role of complexin-lipid interactions in membrane fusion

被引：55

作者：

Seiler, Florian ^{[1
]}

Malsam, Joerg ^{[1
]}

Krause, Jean Michel ^{[1
]}

Soellner, Thomas H. ^{[1
]}

机构：

[1] Univ Heidelberg, Biochem Ctr, D-69120 Heidelberg, Germany

来源：

FEBS LETTERS | 2009年 / 583卷 / 14期

基金：

美国国家卫生研究院;

关键词：

Exocytosis; Fusion; SNARE; NEUROTRANSMITTER RELEASE; DISTINCT DOMAINS; SNARE COMPLEXES; EXOCYTOSIS; PROTEINS; SYNAPTOTAGMIN; BINDING;

D O I：

10.1016/j.febslet.2009.06.025

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Complexins (Cpxs) and synaptotagmins regulate calcium-dependent exocytosis. A central helix in Cpx confers specific binding to the soluble N-ethylmaleimide-sensitive factor-attachment protein receptor (SNARE) fusion machinery. An accessory helix in the amino-terminal region inhibits membrane fusion by blocking SNAREpin zippering. We now show that an amphipathic helix in the carboxy-terminal region of CpxI binds lipid bilayers and affects SNARE-mediated lipid mixing in a liposome fusion assay. The substitution of a hydrophobic amino acid within the helix by a charged residue abolishes the lipid interaction and the stimulatory effect of CpxI in liposome fusion. In contrast, the introduction of the bulky hydrophobic amino acid tryptophan stimulates lipid binding and liposome fusion. This data shows that local Cpx-lipid interactions can play a role in membrane fusion. (C) 2009 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：2343 / 2348

页数：6

共 31 条

[1] Complexin II plays a positive role in Ca2+-triggered exocytosis by facilitating vesicle priming [J].

Cai, Haijiang ;

Reim, Kerstin ;

Varoqueaux, Frederique ;

Tapechum, Sompol ;

Hill, Kerstin ;

Sorensen, Jakob B. ;

Brose, Nils ;

Chow, Robert H. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2008, 105 (49) :19538-19543

[2] How does synaptotagmin trigger neurotransmitter release? [J].

Chapman, Edwin R. .

ANNUAL REVIEW OF BIOCHEMISTRY, 2008, 77 :615-641

[3] Direct interaction of a Ca2+-binding loop of synaptotagmin with lipid bilayers [J].

Chapman, ER ;

Davis, AF .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (22) :13995-14001

[4] Three-dimensional structure of the complexin/SNARE complex [J].

Chen, XC ;

Tomchick, DR ;

Kovrigin, E ;

Araç, D ;

Machius, M ;

Südhof, TC ;

Rizo, J .

NEURON, 2002, 33 (03) :397-409

[5] Conserved structural features of the synaptic fusion complex: SNARE proteins reclassified as Q- and R-SNAREs [J].

Fasshauer, D ;

Sutton, RB ;

Brunger, AT ;

Jahn, R .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (26) :15781-15786

[6] Functional architecture of an intracellular membrane t-SNARE [J].

Fukuda, R ;

McNew, JA ;

Weber, T ;

Parlati, F ;

Engel, T ;

Nickel, W ;

Rothman, JE ;

Söllner, TH .

NATURE, 2000, 407 (6801) :198-202

[7] Distinct domains of complexins bind SNARE complexes and clamp fusion in vitro [J].

Giraudo, Claudio G. ;

Garcia-Diaz, Alejandro ;

Eng, William S. ;

Yamamoto, Ai ;

Melia, Thomas J. ;

Rothman, James E. .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2008, 283 (30) :21211-21219

[8] A clamping mechanism involved in SNARE-dependent exocytosis [J].

Giraudo, Claudio G. ;

Eng, William S. ;

Melia, Thomas J. ;

Rothman, James E. .

SCIENCE, 2006, 313 (5787) :676-680

[9] Alternative Zippering as an On-Off Switch for SNARE-Mediated Fusion [J].

Giraudo, Claudio G. ;

Garcia-Diaz, Alejandro ;

Eng, William S. ;

Chen, Yuhang ;

Hendrickson, Wayne A. ;

Melia, Thomas J. ;

Rothman, James E. .

SCIENCE, 2009, 323 (5913) :512-516

[10] Structure and conformational changes in NSF and its membrane receptor complexes visualized by quick-freeze/deep-etch electron microscopy [J].

Hanson, PI ;

Roth, R ;

Morisaki, H ;

Jahn, R ;

Heuser, JE .

CELL, 1997, 90 (03) :523-535

← 1 2 3 4 →