Inhibition by tributyltin of herring skeletal muscle lactate dehydrogenase activity

被引：8
|
作者：
Gronczewska, J [1 ]
Biegniewska, A [1 ]
Zietara, MS [1 ]
Skorkowski, EF [1 ]
机构：
[1] Univ Gdansk, Biol Stn, PL-80680 Gdansk, Sobieszewo, Poland
来源：
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY | 2004年 / 137卷 / 04期
关键词：
lactate dehydrogenase; LDH-A(4); skeletal muscle; herring; fish; tributyltin; TBT; bovine serum albumin; BSA protection;
D O I：
10.1016/j.cca.2004.02.005
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The activity of herring (Clupea harengus) skeletal muscle lactate dehydrogenase (EC 1.1.1.27) LDH-A(4) isoenzyme was examined in the presence of tributyltin chloride (TBT). This paper reports the in vitro inhibition of LDH activity with increasing concentration of TBT. Bovine serum albumin (BSA) added to the LDH-A(4) isoenzyme prior to the addition of TBT was able to protect enzyme activity against inhibition by this toxicant. The observed protection of LDH-A(4) activity increased with increasing BSA concentration in the incubation medium. The results suggest that the presence of BSA could protect LDH activity from direct binding of TBT to LDH. (C) 2004 Elsevier Inc. All rights reserved.
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页码：307 / 311
页数：5
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