Microfl uidic Bioprinting of Heterogeneous 3D Tissue Constructs Using Low-Viscosity Bioink

被引：700

作者：

Colosi, Cristina ^{[1
,2
,3
]}

Shin, Su Ryon ^{[1
,2
,4
]}

Manoharan, Vijayan ^{[1
,2
]}

Massa, Solange ^{[1
,2
,5
]}

Costantini, Marco ^{[3
]}

Barbetta, Andrea ^{[3
]}

Dokmeci, Mehmet Remzi ^{[1
,2
,4
]}

Dentini, Mariella ^{[3
]}

Khademhosseini, Ali ^{[1
,2
,4
,6
,7
]}

机构：

[1] Harvard Univ, Biomat Innovat Res Ctr, Sch Med, Brigham & Womens Hosp, Cambridge, MA 02139 USA

[2] Harvard MIT Div Hlth Sci & Technol, Cambridge, MA 02139 USA

[3] Univ Roma La Sapienza, Dept Chem, Piazzale Aldo Moro 5, I-00185 Rome, Italy

[4] Wyss Inst Biol Inspired Engn, Boston, MA 02115 USA

[5] Univ Los Andes, Programa Doctorado Biomed, Santiago 7620001, Chile

[6] Konkuk Univ, Dept Bioind Technol, Coll Anim Biosci & Technol, Seoul 143701, South Korea

[7] King Abdulaziz Univ, Dept Phys, Jeddah 21569, Saudi Arabia

来源：

ADVANCED MATERIALS | 2016年 / 28卷 / 04期

基金：

美国国家卫生研究院; 美国国家科学基金会;

关键词：

GELATIN METHACRYLATE; HYDROGELS; COMPLEX; STIFFNESS; PECAM-1; CELLS;

D O I：

10.1002/adma.201503310

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A novel bioink and a dispensing technique for 3D tissue-engineering applications are presented. The technique incorporates a coaxial extrusion needle using a low-viscosity cell-laden bioink to produce highly defined 3D biostructures. The extrusion system is then coupled to a microfluidic device to control the bioink arrangement deposition, demonstrating the versatility of the bioprinting technique. This low-viscosity cell-responsive bioink promotes cell migration and alignment within each fiber organizing the encapsulated cells.

引用

页码：677 / 684

页数：8

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