HIV-1C env and gag Variation in the Cerebrospinal Fluid and Plasma of Patients with HIV-Associated Cryptococcal Meningitis in Botswana

被引:2
作者
Kelentse, Nametso [1 ,2 ]
Moyo, Sikhulile [1 ,3 ]
Mogwele, Mompati L. [1 ,4 ]
Ditshwanelo, Doreen [1 ]
Mokaleng, Baitshepi [1 ,2 ]
Moraka, Natasha O. [1 ,5 ]
Lechiile, Kwana [1 ,6 ]
Leeme, Tshepo B. [1 ,6 ]
Lawrence, David S. [1 ,7 ]
Musonda, Rosemary [1 ,3 ]
Kasvosve, Ishmael [2 ]
Harrison, Thomas S. [8 ]
Jarvis, Joseph N. [1 ,6 ,7 ,9 ]
Gaseitsiwe, Simani [1 ,3 ]
机构
[1] Botswana Harvard AIDS Inst Partnership, Gaborone, Botswana
[2] Univ Botswana, Fac Hlth Sci, Sch Allied Hlth Profess, Gaborone, Botswana
[3] Harvard TH Chan Sch Publ Hlth, Dept Immunol & Infect Dis, Boston, MA 02115 USA
[4] Univ Botswana, Dept Biol Sci, Gaborone, Botswana
[5] Stellenbosch Univ, Dept Pathol, ZA-7505 Stellenbosch, South Africa
[6] Botswana Univ Pennsylvania Partnership, Gaborone, Botswana
[7] London Sch Hyg & Trop Med, Fac Infect & Trop Dis, Dept Clin Res, London WC1E 7HT, England
[8] St Georges Univ London, Inst Infect & Immun, Ctr Global Hlth, London SW17 0RE, England
[9] Univ Penn, Perelman Sch Med, Dept Med, Div Infect Dis, Philadelphia, PA 19104 USA
来源
VIRUSES-BASEL | 2020年 / 12卷 / 12期
基金
英国惠康基金;
关键词
Botswana; CCR5; CXCR4; cerebrospinal fluid; co-receptor; cryptococcal meningitis; escape mutations; human immunodeficiency virus (HIV); plasma; LINKED GLYCOSYLATION SITE; ANTIRETROVIRAL THERAPY; GLYCAN SHIELD; ESCAPE; TROPISM; RESERVOIRS; MUTATIONS; INDIVIDUALS; PERFORMANCE; ALGORITHMS;
D O I
10.3390/v12121404
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
HIV-1 compartmentalization in reservoir sites remains a barrier to complete HIV eradication. It is unclear whether there is variation in HIV-1 env and gag between cerebrospinal fluid (CSF) and plasma of individuals with HIV-associated cryptococcal meningitis (CM). We compared HIV-1 env characteristics and the gag cytotoxic T-lymphocyte (CTL) escape mutations from CSF and plasma samples. Employing population-based Sanger sequencing, we sequenced HIV-1 env from CSF of 25 patients and plasma of 26 patients. For gag, 15 CSF and 21 plasma samples were successfully sequenced. Of these, 18 and 9 were paired env and gag CSF/plasma samples, respectively. There was no statistically significant difference in the proportion of CCR5-using strains in the CSF and plasma, (p = 0.50). Discordant CSF/plasma virus co-receptor use was found in 2/18 pairs (11.1%). The polymorphisms in the HIV-1 V3 loop were concordant between the two compartments. From the HIV-1 gag sequences, three pairs had discordant CTL escape mutations in three different epitopes of the nine analyzed. These findings suggest little variation in the HIV-1 env between plasma and CSF and that the CCR5-using strains predominate in both compartments. HIV-1 gag CTL escape mutations also displayed little variation in CSF and plasma suggesting similar CTL selective pressure.
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页数:17
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