Aggression in imported fire ants: an explanation for shifts in their spatial distributions in Southern United States?

1. The imported fire ants, Solenopsis invicta Buren (red), S. richteri Forel (black), and their hybrids (S. invicta x S. richteri) are sympatric congeners with overlapping but shifting spatial distributions in northern Alabama and Mississippi, United States. 2. The abilities of workers of the three ant forms (or species) to recognise non-nestmate conspecifics and heterospecifics were compared in separate aggression bioassays using three group sizes of competitor numbers (battles): 1-1, 5-1, and 5-5 resident-intruder battles. 3. Workers of all three forms showed significant aggression toward non-nestmate conspecifics and heterospecifics. The results of the intra-specific aggression bioassays showed that S. invicta had the lowest aggression threshold, whereas S. richteri was the least aggressive. Survival rates 1 day after intra-specific encounters were higher for S. richteri than for S. invicta or hybrids, consistent with its lower aggressiveness. 4. In inter-specific interactions, S. invicta workers showed the greatest aggression and were more aggressive towards S. richteri than to hybrids. Furthermore, survival 1 day after inter-specific encounters was highest for S. invicta workers, whether they were the intruders or residents. The hybrid form was intermediate between both parental forms in aggression and post-aggression survival. 5. The live 1-1 and 5-5 battles produced similar and consistent results, but differences were amplified in the 5-5 battles, suggesting an effect of group size on aggression. These results may explain the observed shifts in the spatial distributions of the three forms and range restriction of S. richteri in southern United States.