Protein condensates as aging Maxwell fluids

被引：261

作者：

Jawerth, Louise ^{[1
,2
]}

Fischer-Friedrich, Elisabeth ^{[3
,4
]}

Saha, Suropriya ^{[1
,8
]}

Wang, Jie ^{[2
,9
]}

Franzmann, Titus ^{[2
,4
]}

Zhang, Xiaojie ^{[5
]}

Sachweh, Jenny ^{[5
,10
]}

Ruer, Martine ^{[2
]}

Ijavi, Mandiye ^{[2
,11
]}

Saha, Shambaditya ^{[6
]}

Mahamid, Julia ^{[5
]}

Hyman, Anthony A. ^{[2
,3
,7
]}

Juelicher, Frank ^{[1
,3
,7
]}

机构：

[1] Max Planck Inst Phys Komplexer Syst, Nothnitzer St 3, D-01187 Dresden, Germany

[2] Max Planck Inst Mol Cell Biol & Genet, Pfotenhauerstr 108, D-01307 Dresden, Germany

[3] Tech Univ Dresden, Cluster Excellence Phys Life, Dresden, Germany

[4] Tech Univ Dresden, Biotec, Tatzberg 47-49, D-01307 Dresden, Germany

[5] EMBL Heidelberg, Meyerhofstr 1, D-69117 Heidelberg, Germany

[6] Austrian Acad Sci IMBA, Inst Mol Biotechnol, Vienna BioCtr VBC, Dr Bohrgasse 3, A-1030 Vienna, Austria

[7] Ctr Syst Biol Dresden, Pfotenhauerstr 108, D-01307 Dresden, Germany

[8] Max Planck Inst Dynam & Selforg, Fassberg 17, D-37077 Gottingen, Germany

[9] Whitehead Inst Biomed Res, 455 Main St, Cambridge, MA 02142 USA

[10] Max Planck Inst Biophys, Max von Laue Str 3, D-60438 Frankfurt, Germany

[11] Swiss Fed Inst Technol, Vladimir Prelog Weg 1-5-10, CH-8093 Zurich, Switzerland

来源：

SCIENCE | 2020年 / 370卷 / 6522期

基金：

欧洲研究理事会;

关键词：

PHASE-SEPARATION; BROWNIAN-MOTION; LIQUID DROPLETS; TRANSITION; RHEOLOGY; GRANULES; DYNAMICS;

D O I：

10.1126/science.aaw4951

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Protein condensates are complex fluids that can change their material properties with time. However, an appropriate rheological description of these fluids remains missing. We characterize the time-dependent material properties of in vitro protein condensates using laser tweezer-based active and microbead-based passive rheology. For different proteins, the condensates behave at all ages as viscoelastic Maxwell fluids. Their viscosity strongly increases with age while their elastic modulus varies weakly. No significant differences in structure were seen by electron microscopy at early and late ages. We conclude that protein condensates can be soft glassy materials that we call Maxwell glasses with age-dependent material properties. We discuss possible advantages of glassy behavior for modulation of cellular biochemistry.

引用

页码：1317 / +

页数：40

共 60 条

[1] Probing a nonequilibrium Einstein relation in an aging colloidal glass [J].