Coupling Microdroplet Microreactors with Mass Spectrometry: Reading the Contents of Single Droplets Online

被引：140
作者：
Fidalgo, Luis M. [1 ]
Whyte, Graeme [1 ]
Ruotolo, Brandon T.
Benesch, Justin L. P.
Stengel, Florian
Abell, Chris
Robinson, Carol V.
Huck, Wilhelm T. S. [1 ]
机构：
[1] Univ Cambridge, Dept Chem, Melville Lab Polymer Synth, Cambridge CB2 1EW, England
来源：
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2009年 / 48卷 / 20期
基金：
英国工程与自然科学研究理事会;
关键词：
fluorescence screening; high-throughput screening; mass spectrometry; microdroplets; microfluidics; MICROFLUIDICS; SYSTEMS; TIME; NANOPARTICLES; ENCAPSULATION; VOLUME; SCALE; CHIP;
D O I：
10.1002/anie.200806103
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Fully integrated: Mass spectrometry has been integrated into a detection scheme for microdroplets that are created within microfluidic channels (see picture, scale bar 200 μm). This technique allows droplets to be identified based on the compounds they contain, and combines fluorescence screening with MS analysis. These experiments indicate how similar approaches can be applied to the ambitious goals of on-chip protein evolution and chemical synthesis. © 2009 Wiley-VCH Verlag GmbH & Co. KGaA.
引用
收藏
页码：3665 / 3668
页数：4
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