Direct electrochemistry and electrocatalysis of hemoglobin immobilized in an amphiphilic diblock copolymer film

被引：23

作者：

Jia, Shanshan ^{[1
]}

Fei, Junjie ^{[1
]}

Deng, Junjie ^{[1
]}

Cai, Yuanli ^{[1
]}

Li, Junan ^{[1
]}

机构：

[1] Xiangtan Univ, Coll Chem, Minist Educ, Key Lab Environm Friendly Chem & Applicat, Xiangtan 411105, Peoples R China

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 2009年 / 138卷 / 01期

基金：

中国国家自然科学基金;

关键词：

Hemoglobin; Amphiphilic block copolymer; Direct electrochemistry; Nitric oxide; Electrocatalysis; DIRECT ELECTRON-TRANSFER; REDUCTION;

D O I：

10.1016/j.snb.2008.12.009

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A well-defined amphiphilic diblock copolymer, poly [N-(2-methacryloyloxyethyl) pyrrolidone]-block-poly [glycidyl methacrylate] (PNMP-b-PGMA), was applied to immobilize hemoglobin (Hb) in a simple procedure. The characterization of PNMP-b-PGMA/Hb film was demonstrated by ultraviolet-visible (UV-vis) spectra, atomic force microscopy (AFM), and electrochemical impedance spectroscopy (EIS). The electrochemical behaviors of Hb in PNMP-b-PGMA film have been investigated and a pair of well-defined and nearly reversible cyclic voltammetric peaks for the protein heme Fe(III)/Fe(II) redox couples were observed at about -0.409V (vs. SCE) in pH 7.0 phosphate buffer solution (PBS). The results showed that the PNMP-b-PGMA film improved the protein loading with the retention of bioactivity and greatly promoted the direct electron transfer between Hb and electrode surface. This PNMP-b-PGMA/Hb film modified electrode also exhibited high electrocatalytic effects towards reduction of nitric oxide (NO). These results indicated the potential applicability of the films as new types of third-generation biosensors or bioreactors based on direct electrochemistry of the proteins. (C) 2008 Elsevier B.V. All rights reserved.

引用

页码：244 / 250

页数：7