On-line HPLC detection of tocopherols and other antioxidants through the formation of a phosphomolybdenum complex

被引:15
作者
Cardeñosa, R
Mohamed, R
Pineda, M
Aguilar, M
机构
[1] Univ Cordoba, Dept Bioquim & Biol Mol, Cordoba 14071, Spain
[2] Univ Cordoba, Inst Andaluz Biotechnol, Cordoba 14071, Spain
关键词
antioxidants; tocopherols; on-line detection; HPLC (high-performance liquid chrmatography); molybdenum; phosphate;
D O I
10.1021/jf011489y
中图分类号
S [农业科学];
学科分类号
09 ;
摘要
An on-line method to detect and quantify antioxidant species in complex extracts has been developed as a combination of conventional HPLC separation and a postcolumn reaction with phosphomolybdenum reagent at acidic pH. Sample analytes were chromatographed by HPLC, and the postcolumn formation of a phosphate/Mo(V) complex was detected at 598 nm with an on-line absorbance detector. An optimized instrumental system was set up using pure alpha-tocopherol, and it was successfully tested with complex food extracts including lettuce, tomato, red pepper, and soybean seed, where several tocopherols and carotenoids were identified. A potential application of this detection method to quantitatively determine different antioxidants was considered, and a specific application to the determination of tocopherols was developed. The new method was characterized with respect to linearity interval, repeatability, and reproducibility, the quantitative results obtained were validated by comparison with a conventional HPLC method with fluorometric detection, and it was applied to the determination of tocopherols in different foods. The results suggest that the proposed on-line HPLC method can be a powerful instrument for the detection, purification, and characterization of natural antioxidants.
引用
收藏
页码:3390 / 3395
页数:6
相关论文
共 29 条
[21]   Antioxidative activity of natural products from plants [J].
Ng, TB ;
Liu, F ;
Wang, ZT .
LIFE SCIENCES, 2000, 66 (08) :709-723
[22]  
PASSEY RB, 1996, EP6P NCCLS, V6, P18
[23]   Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: Specific application to the determination of vitamin E [J].
Prieto, P ;
Pineda, M ;
Aguilar, M .
ANALYTICAL BIOCHEMISTRY, 1999, 269 (02) :337-341
[24]   Measurement of the antioxidant status of dietary constituents, low density lipoproteins and plasma [J].
RiceEvans, C ;
Miller, N .
PROSTAGLANDINS LEUKOTRIENES AND ESSENTIAL FATTY ACIDS, 1997, 57 (4-5) :499-505
[25]  
SIMS RJ, 1991, BIOTECHNOLOGY FOOD I
[26]   CHIA SEEDS AS A SOURCE OF NATURAL LIPID ANTIOXIDANTS [J].
TAGA, MS ;
MILLER, EE ;
PRATT, DE .
JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY, 1984, 61 (05) :928-931
[27]   Spectrophotometric determination of vitamin E (alpha-tocopherol) using copper(II)-neocuproine reagent [J].
Tutem, E ;
Apak, R ;
Gunaydi, E ;
Sozgen, K .
TALANTA, 1997, 44 (02) :249-255
[28]  
Valkonen M, 1997, J LIPID RES, V38, P823
[29]  
VISIOLI F, 1995, EXPERIENTIA, V51, P32